CYTOMORPHOLOGICAL, CYTOCHEMICAL, CYTOENZYMIC AND ULTRASTRUCTURAL STUDIES ON BLOOD CELLS OF EMU
Loading...
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Birsa Agricultural University, Ranchi
Abstract
The present study was conducted on blood cells of twelve healthy adult emu birds. The
formed element includes erythrocytes, leukocytes and thrombocytes. The leucocytes can be further
classified into granulocytes (neutrophils, eosinophils and basophils) and agranulocytes
(lymphocytes and monocytes).
The erythrocytes were nucleated, oval to elongated in shape and were 15.20 ± 0.16μm in
length and 8.11 ± 0.18m in width. The nuclei were centrally placed and rounded, oval to elongated
in shape. The heterophils were almost rounded to spherical in shape and measured 12.53 ± 0.14 m
in diameter. The nuclei were mostly 2-4 lobed and varied in shape, size and number. Nuclei were
eccentrically placed and connected with very thin and fine strands. The eosinophils were largest in
size and slightly elongated to round in shape with size of 12.78 ± 0.13 m in diameter. The nuclei
of eosinophils were eccentrically placed and mostly bilobed. The cytoplasmic granules were
strongly eosinophilic which were pink in colour, large, round, numerous and uniformly distributed
throughout the cytoplasm. The basophils were pear to oval in shape and 12.66 ± 0.19 m in
diameter. The nuclei of basophils were partially visible as the cytoplasm was filled with numerous
dark, rounded bluish- purple color basophilic granules mostly concentrated at periphery. Most
common lymphocytes were small sized lymphocytes with mean diameter of 6.19 ± 0.15 m. The
medium sized lymphocytes were almost rounded in shape and 8.45 ± 0.23 m in size. The large
sized lymphocytes were 10.00 ± 0.18 m in diameter. The monocytes were roughly rounded in
shape and measured 12.01 ± 0.36 m in diameter. The cytoplasm was foamy in appearance due to
presence of large number of vacuoles in monocytes. The thrombocytes were oval to round in
shape and 7.10 ± 0.20 m in diameter. The blood smears of emu were firstly stained with Toluidine
Description
CYTOMORPHOLOGICAL, CYTOCHEMICAL,
CYTOENZYMIC AND ULTRASTRUCTURAL
STUDIES ON BLOOD CELLS OF EMU