A Study on the Enteropathy of Salmonellae and Their Enterotoxins
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Date
1993
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Birsa Agricultural University, Ranchi
Abstract
All the serotypes of Salmonella showed vascular permeability reactions (VPR) on rabbit skin. Therefore, it was concluded that VPR test might be employed as an alternative assay system for screening enterotoxigenic Salmonelise.
There was variation in the heat stability of the enterotoxins of S ohlo, S. bornum and Salmonella (3:10:r:-). While the CFS of S. ohfo induced enough fluid accumulation in RLIL to consider it positive for enterotoxic activity after heating at 70°c for 30 minutes the CPS of S bornum and Salmonella (3:10:r:-) showed decrease in the enterotoxicity activity at 70°C for 30 minutes. However, the CFS of all the serotypes of Salmonella were found to be heat labile and lost their enterotoxicity at 80°C for 30 minutes.
The enterotoxic activity of the CFS of all the Salmonellae maintained between pH 6.0 and 8.0.
The enterotoxins were sensitive to proteolytic enzyme trypsin.
The enterotoxins present in CFS of the organisms were salt precipitable with ammonium sulphate at 60.0 per cent saturation level.
It was concluded that enteropathy of diarrhoea associated with Sohio, S. bornum and Salmonella (3:10:r:-) was enterotoxin mediated.