Evaluation of pheromone-based kit: A noninvasive approach of estrus detection in buffalo
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Date
2018-07
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Abstract
In view of the silent nature of estrus in buffalo, a noninvasive assay kit has long been
felt necessary for easy and effective estrus detection. This study was designed to
detect estrus in buffalo using a kit formulated in our laboratory based on pheromone
compound. Group I: Urine samples collected at estrus phase and group II: randomly
collected urine samples were subjected to the test using the kit. No colour developed
(i.e., positive reaction) in estrus urine after adding the kit solution. By contrast, pale
and/or dark pink colour developed (i.e., negative reaction) in urine from the proestrus
and diestrus buffaloes, respectively. Field evaluation of the kit in groups I and II revealed
that 60.87% and 71.43% of urine samples were correctly identified as estrus
and nonestrus (i.e., proestrus and diestrus), respectively. Therefore, the first of its
kind estrus detection kit formulated based on urinary pheromone can as well be used
as a simple device to detect estrus in buffalo.
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Keywords
Veterinary Science, Veterinary Gynaecology and Obstetrics