DEVELOPMENT OF In Vitro SYSTEMS OF Colleus forskohlii FOR FORSKOLIN PRODUCTION

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dc.contributor.advisorBhattacharya, Amita
dc.contributor.authorSharma, Mohini
dc.date.accessioned2019-08-02T08:55:46Z
dc.date.available2019-08-02T08:55:46Z
dc.date.issued2019-07-06
dc.description.abstractThe roots and tubers of Coleus forskohlii,, an endangered herb of family,, Lamiaceae are medicinally important because of the presence of forskolin,, an alkaloid popular for its anttii-obese,, bllood-pressure-lloweriing,, nerve-regenerattiing and iinttraocullarpressure lloweriing properttiies.. Owiing tto adenyllatte-cycllase-sttiimullantt acttiiviitty,, forskolliin iis used iin ttreattiing gllaucoma,, congesttiive-cardiiomyopatthy and astthma.. Indiiscriimiinatte up-roottiing of tthe pllantt for forskolliin has lled tto acutte scarciitty of raw matteriiall.. There are no cullttiivattiion practtiices and tthe forskolliin llevells are allso llow iin rootts and ttubers.. Therefore,, an objjecttiive of tthe tthesiis was tthe devellopmentt of miicropropagattiion prottocolls for UP NBRC-10,, UP NBRC-13,, J&K,, Tamiillnadu,, Punjjab and Haryana accessiions of C.. fforskohlliiii.. Resulltts revealled maxiimum shoott mullttiiplliicattiion (approx.. 70 shootts) occurred on 0..8% agar gelllled (w//v) fullll sttrengtth MS mediium conttaiiniing 3% sucrose,, 7..50 μM BAP and 2..50 μM IBA butt shoott ellongattiion was maxiimum on 3% sucrose suppllementted fullll sttrengtth PGR-free-MS mediium.. In comparattiive growtth performance Haryana accessiion supportted maxiimum number of rootts,, whiille Tamiillnadu had maxiimum roott llengtth.. In hardeniing experiimentt,, maxiimum surviivall was recorded iin sand duriing summer.. When variious auxiins were used,, maxiimum callllusiing wiitth miiniimum rhiizogenesiis occurred iin lleaf and sttem expllantts of UP NBRC-13 and J&K att 10 μM 2,,4-D.. Adventtiittiious roott formattiion was maxiimum iin lleaves of Haryana accessiion on 2..5μM NAA suppllementted 0..8% agar gelllled and lliiquiid MS mediia.. In tthe second objjecttiive,, tthe forskolliin conttentt iin rootts,, sttem and lleaves of iin viittro and fiielld grown accessiions were measured usiing HPTLC//UHPLC.. Forskolliin was hiighestt iin rootts of iin viittro grown Haryana accessiion.. In case of adventtiittiious rootts,, forskolliin was maxiimum iin Tamiillnadu butt llow iin Hayana accessiion.. Ten ttiimes hiigher iincrease was recorded att 12..5 μM IBA.. The fiindiings pave tthe way for en masse propagattiion of hiigh-forskolliin-yiielldiing C.. fforskohlliiii pllantts for iindusttriiall use.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810118624
dc.keywordsColeus forskohliien_US
dc.language.isoenen_US
dc.pages122en_US
dc.publisherCSKHPKV, Palampuren_US
dc.subAgricultural Biotechnologyen_US
dc.subjectnullen_US
dc.themeIdentification of high forskolin yielding genotypes.en_US
dc.these.typeM.Scen_US
dc.titleDEVELOPMENT OF In Vitro SYSTEMS OF Colleus forskohlii FOR FORSKOLIN PRODUCTIONen_US
dc.typeThesisen_US
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