DEVELOPMENT OF In Vitro SYSTEMS OF Colleus forskohlii FOR FORSKOLIN PRODUCTION
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Date
2019-07-06
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CSKHPKV, Palampur
Abstract
The roots and tubers of Coleus forskohlii,, an endangered herb of family,, Lamiaceae
are medicinally important because of the presence of forskolin,, an alkaloid popular
for its anttii-obese,, bllood-pressure-lloweriing,, nerve-regenerattiing and iinttraocullarpressure
lloweriing properttiies.. Owiing tto adenyllatte-cycllase-sttiimullantt acttiiviitty,, forskolliin
iis used iin ttreattiing gllaucoma,, congesttiive-cardiiomyopatthy and astthma.. Indiiscriimiinatte
up-roottiing of tthe pllantt for forskolliin has lled tto acutte scarciitty of raw matteriiall.. There
are no cullttiivattiion practtiices and tthe forskolliin llevells are allso llow iin rootts and ttubers..
Therefore,, an objjecttiive of tthe tthesiis was tthe devellopmentt of miicropropagattiion
prottocolls for UP NBRC-10,, UP NBRC-13,, J&K,, Tamiillnadu,, Punjjab and Haryana
accessiions of C.. fforskohlliiii.. Resulltts revealled maxiimum shoott mullttiiplliicattiion (approx..
70 shootts) occurred on 0..8% agar gelllled (w//v) fullll sttrengtth MS mediium conttaiiniing
3% sucrose,, 7..50 μM BAP and 2..50 μM IBA butt shoott ellongattiion was maxiimum on
3% sucrose suppllementted fullll sttrengtth PGR-free-MS mediium.. In comparattiive growtth
performance Haryana accessiion supportted maxiimum number of rootts,, whiille
Tamiillnadu had maxiimum roott llengtth.. In hardeniing experiimentt,, maxiimum surviivall
was recorded iin sand duriing summer.. When variious auxiins were used,, maxiimum
callllusiing wiitth miiniimum rhiizogenesiis occurred iin lleaf and sttem expllantts of UP
NBRC-13 and J&K att 10 μM 2,,4-D.. Adventtiittiious roott formattiion was maxiimum iin
lleaves of Haryana accessiion on 2..5μM NAA suppllementted 0..8% agar gelllled and
lliiquiid MS mediia.. In tthe second objjecttiive,, tthe forskolliin conttentt iin rootts,, sttem and
lleaves of iin viittro and fiielld grown accessiions were measured usiing HPTLC//UHPLC..
Forskolliin was hiighestt iin rootts of iin viittro grown Haryana accessiion.. In case of
adventtiittiious rootts,, forskolliin was maxiimum iin Tamiillnadu butt llow iin Hayana
accessiion.. Ten ttiimes hiigher iincrease was recorded att 12..5 μM IBA.. The fiindiings pave
tthe way for en masse propagattiion of hiigh-forskolliin-yiielldiing C.. fforskohlliiii pllantts for
iindusttriiall use.
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