STUDIES ON PROLIFERATION BIOMARKERS IN SQUAMOUS CELL CARCINOMA OF BULLOCK HORN BY IMMUNOHISTOCHEMISTRY AND ITS CORRELATION WITH HISTOPATHOLOGY, 2996
dc.contributor.advisor | D. T. FEFAR | |
dc.contributor.author | CHAUHAN JUHIBEN MANSUKHBHAI | |
dc.date.accessioned | 2021-06-19T11:26:23Z | |
dc.date.available | 2021-06-19T11:26:23Z | |
dc.date.issued | 2019-09 | |
dc.description.abstract | The present study was undertaken to evaluate the expression of proliferation biomarkers in squamous cell carcinoma of bullock horn by immunohistochemistry and to correlate with histopathological study. A total of twenty horn cancer tissue samples were collected from bullock from Teaching Veterinary Clinical Complex (TVCC) of College of Veterinary Science & A. H., JAU, Junagadh, Gujarat as well as from the field. The primary information pertaining to case viz. sex, breed, age and side of affected horn were collected. Pre-operatively, clinical observation of the horn cancer were carried out. Gross pathological lesions of cancerous growth were recorded after surgical amputation of horn. Subsequently, affected tissue samples as well as tissue of normal horn core were collected and fixed in 10% neutral-buffered formalin and later processed for histopathological confirmation of squamous cell carcinoma. Further, histopathologically confirmed SCC tissues were subjected to grading of squamous cell carcinoma based on cytoplasmic keratinization, nuclear pleomorphism, mitotic figure, mode of invasion and cellular response. Subsequently, expression study of proliferative biomarkers viz., Ki-67, PCNA and cyclin D1 were also carried out in histopathologically confirmed and graded squamous cell carcinoma as well as in normal horn core by immunohistochemistry. In present the study, the frequency of occurrence of horn cancer cases was highest in the age group of 6 to 10 years (70%) followed by 10 to 15 years (30%). Majority of the cases (70%) were recorded between 6 to 10 years of age. The breed wise frequency of horn cancer in randomly selected twenty cases under study stated that the frequency of occurrence of horn cancer was highest in Kankrej 13/20 (65%) followed by Gir 6/20 (30%) and non-descript 1/20 (5%) breeds of cattle. Clinical observations found in horn cancer cases were frequent shaking of head, tilting of head at the affected side, tilting of affected horn and increase nasal discharge from affected side in advance cases. Gross examination of surgical amputation of affected horn revealed cancerous growth in the middle and distal region of the horn core in first stage of horn cancer. Histopathological examination of affected tissue from this region revealed the well-differentiated squamous cell carcinoma in five cases while low differentiated squamous cell carcinoma revealed in one case. In second stage of horn cancer, cancerous tissue found nearly in entire horn core, that was dry, hard and dirty grey in color, and revealed the well-differentiated squamous cell carcinoma in four cases as well as low differentiated squamous cell carcinoma in two cases in histopathological examination of all the affected tissue from this region. Third stage of horn cancer showed completely filled horn core with cauliflower like cancerous growth and histopathological examination of all the affected tissue from this region revealed the well-differentiated squamous cell carcinoma in one case as well as low differentiated squamous cell carcinoma in seven cases. Histopathologically, all collected samples found positive for squamous cell carcinoma of horn core, which further classify into well-differentiated and poorly differentiated SCC. Well differentiated SCC revealed higher cytoplasmic keratinization, low nuclear differentiation, less mitotic figures, well defined border line and mild cellular response. The low cytoplasmic keratinization, high nuclear differentiation, high mitotic figures, poorly defined border line and high cellular response were found in poorly differentiated SCC. Immunohistochemically expression study of Ki-67 and PCNA revealed showed strong immunorecativity in low differentiated SCC and weak immunoreactivity in well-differentiated SCC and normal horn core tissue. The expression of Ki-67 and PCNA significantly found higher in poorly differentiated SCC compared to well-differentiated SCC. The expression study of cyclin D1 could not be appreciated in any of the squamous cell carcinoma as well as in normal horn tissue. Hence, the present study suggested that analysis of Ki-67 and PCNA expression in tumoral tissue may help to predict the clinical course and prognosis in patients with squamous cell carcinoma. | en_US |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810169886 | |
dc.keywords | Horn cancer, Histopathology, Grading of squamous cell carcinoma, Immunohistochemistry. | en_US |
dc.language.iso | English | en_US |
dc.pages | 100p | en_US |
dc.publisher | JAU, JUNAGADH | en_US |
dc.sub | Veterinary Pathology | en_US |
dc.theme | STUDIES ON PROLIFERATION BIOMARKERS IN SQUAMOUS CELL CARCINOMA OF BULLOCK HORN BY IMMUNOHISTOCHEMISTRY AND ITS CORRELATION WITH HISTOPATHOLOGY, 2996 | en_US |
dc.these.type | M.V.Sc. | en_US |
dc.title | STUDIES ON PROLIFERATION BIOMARKERS IN SQUAMOUS CELL CARCINOMA OF BULLOCK HORN BY IMMUNOHISTOCHEMISTRY AND ITS CORRELATION WITH HISTOPATHOLOGY, 2996 | en_US |
dc.type | Thesis | en_US |
Files
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- 2996.pdf
- Size:
- 7.68 MB
- Format:
- Adobe Portable Document Format
- Description:
- STUDIES ON PROLIFERATION BIOMARKERS IN SQUAMOUS CELL CARCINOMA OF BULLOCK HORN BY IMMUNOHISTOCHEMISTRY AND ITS CORRELATION WITH HISTOPATHOLOGY, 2996
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: