EFFICACY OF ZINC OXIDE (ZnO) NANOPARTICLES IN DIETHYLNITROSAMINE (DEN) INDUCED HEPATOCARCINOGENESIS IN RATS
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Date
2018-07
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KARNATAKA VETERINARY, ANIMAL & FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The present study was carried out to determine the prophylactic and therapeutic
anticancerous efficacy of Zinc oxide nanoparticles (ZnO NPs) against DEN induced
hepatocarcinogenesis in rats. The various groups in the study included negative control
(Group I), DEN control (Group II), ZnO NPs control (Group III), ZnO NPs and DEN
concurrent group (Group IV), ZnO NPs pre-treatment group (Group V) and ZnO NPs
post-treatment group (Group VI). Hepatocarcinogenesis was induced by single
intraperitoneal injection of DEN (100 mg/kg) on Day one followed by 0.01 % DEN in
drinking water ad libitum throughout the study period. Induction of hepatocarcinogenesis
was determined by the appearance of grayish white nodules on the surface of liver
grossly and confirmed by microscopical examination. ZnO NPs were used at a dose rate
of 25 mg/kg body weight. The effect of DEN administration and ZnO NPs treatment in
different groups were assessed by hemato-biochemical parameters, oxidant and
antioxidant prolife, histopathology, immunohistochemical and molecular evaluation of
p53 and glutamine synthetase expression. DEN induced hepatocarcinogenesis as early as
by 60th day and induced larger growths of hepatocellular carcinoma by 90th day. ZnO NPs
concurrent and pre-treatment delayed the onset of DEN induced hepatocarcinogenesis
and reduced the number of hepatocellular carcinoma nodules. ZnO NPs post-treatment
did not reveal any effect on completely developed hepatic tumors. Based on the results, it
was concluded that ZnO NPs have prophylactic effect but no therapeutic effect against
DEN induced hepatocarcinogenesis.