STUDIES ON INDUCED METAGENESIS AND in vitro REGENERATION IN TURMERIC {Curcuma longa L.)
| dc.contributor.author | RAMAKRISHNAV. HEGDE | |
| dc.contributor.other | A. N. MOKASHI | |
| dc.date.accessioned | 2019-06-21T05:31:45Z | |
| dc.date.available | 2019-06-21T05:31:45Z | |
| dc.date.issued | 2007-08-31 | |
| dc.description.abstract | Turmeric is an important rhizomatous spice, combining properties of colourant, cosmetic and drug. Sterile triploid nature of turmeric is one of the bottlenecks in crop improvement. Being a vegetatively propagated crop, mutation breeding is an important tool in turmeric improvement. Studies were conducted to standardize the optimum dose of mutagen i.e., gamma irradiation for in vivo and chemical mutagens for in vitro raised plants, to standardize the protocol for in vitro clonal propagation and to study the variability in the induced mutants. Among the two cultivars evaluated, Salem was more sensitive to gamma irradiation with a LD50 dose of 1.135 kR compared to Cuddappah (2.69 kR). Mutant spectrum showed predominance of striata type and dwarf types in chlorophyll and morphological mutants respectively. The study on vegetative characters indicated that lower doses of 0.5 and 1.0 kR did not affect the characters significantly. The vegetative characters and crop duration was drastically reduced at 5.0 kR and as a result, plants did not form the rhizomes. Higher degree of variability was observed for characters such as number of tillers, number of leaves, leaf area, weight of mother and finger rhizomes. The highest GCV and PCV was observed for weight of finger rhizomes. C-1.0 - 10/4 (546.9g) and S-0.5-9/3 (487.48) were selected for higher rhizome yield, S-0.5 - 9/9 (24.7%) for recovery and SO.5-6/1 (5.42) for higher curcumin content after evaluating evaluation of M1V2 progenies. In vitro studies for clonal propagation of turmeric indicated that the highest number of multiple shoots in turmeric was obtained in MS medium supplemented with 2.0 mg/1 BAP and 0.2 mg/1 NAA whereas, better rooting was obtained with 0.5 mg/1 IB A. The in vitro mutation studies in cv. Salem with chemical mutagens indicated that the LD50 for EMS was 114.58 |iM and DES was 28.80 pM. Both mutagens increased the variability in vegetative and reproductive characters. Mutants D-1-3 (318.25g) and E-200-4 (285.83g) gave the highest rhizome yield whereas, D-10-3 recorded the highest curcumin content. | en_US |
| dc.identifier.other | TH8821 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810109428 | |
| dc.language.iso | en | en_US |
| dc.pages | 186 | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Banglore | en_US |
| dc.sub | Horticulture | en_US |
| dc.subject | null | en_US |
| dc.theme | STUDIES ON INDUCED METAGENESIS AND in vitro REGENERATION IN TURMERIC {Curcuma longa L.) | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | STUDIES ON INDUCED METAGENESIS AND in vitro REGENERATION IN TURMERIC {Curcuma longa L.) | en_US |
| dc.type | Thesis | en_US |