Pathological and Molecular Diagnosis of Paratuberculosis in Sheep and Goats

Simple item page
dc.contributor.authorKanimozhi, P
dc.contributor.authorGeetha, M
dc.contributor.authorPalanivel, KM, et al.
dc.contributor.authorTANUVAS
dc.date.accessioned2020-02-04T10:28:46Z
dc.date.available2020-02-04T10:28:46Z
dc.date.issued2018
dc.descriptionTNV_IJCMAS_2018_7(8)2360-2363en_US
dc.description.abstractJohne’s disease (JD) is caused by Mycobacterium avium Subsp. Paratuberculosis (MAP) and the disease is characterized by chronic diarrhea, progressive weight loss and emaciation. The present study was carried out to find out the MAP infection status by postmortem and molecular diagnosis in sheep and goats suspected for JD. Postmortem examination of six small ruminants (One sheep and five goats) revealed the pathognomonic necrotic findings such as characterised hypertrophy of mucosal surfaces of small intestine (terminal ileum) and cord like thickening of mesenteric lymphnodes. Acid fast staining of cytological smears prepared from the intestinal scrapings and mesenteric lymphnode of all animals revealed presence of clusters of acid fast bacilli. Mesentric lymphnode and intestine were collected from during postmortem examination and subjected to polymerase chain reaction (PCR) using specific primers targeting IS 900 gene sequence of MAP which revealed positivity to MAP infection. The study concluded that postmortem examination combined with polymerase chain reaction is highly useful in accurate diagnosis of JD in small ruminants.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810142454
dc.keywordsJohne's disease, Mycobacterium Avium Subsp. Paratuberculosis, PCR, Sheep and goatsen_US
dc.language.isoenen_US
dc.pages2360-2363en_US
dc.relation.ispartofseries;8
dc.subjectVeterinary Scienceen_US
dc.titlePathological and Molecular Diagnosis of Paratuberculosis in Sheep and Goatsen_US
dc.title.alternativeInt.J.Curr.Microbiol.App.Sci.en_US
dc.typeArticleen_US
dc.volume7en_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
TNV_IJCMAS_2018_7(8)2360-2363.pdf
Size:
219.27 KB
Format:
Adobe Portable Document Format
Description:
TNV_IJCMAS_2018_7(8)2360-2363
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Articles (1.Journal)