Pathological and Molecular Diagnosis of Paratuberculosis in Sheep and Goats
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Date
2018
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Abstract
Johne’s disease (JD) is caused by Mycobacterium avium Subsp. Paratuberculosis (MAP)
and the disease is characterized by chronic diarrhea, progressive weight loss and
emaciation. The present study was carried out to find out the MAP infection status by
postmortem and molecular diagnosis in sheep and goats suspected for JD. Postmortem
examination of six small ruminants (One sheep and five goats) revealed the
pathognomonic necrotic findings such as characterised hypertrophy of mucosal surfaces of
small intestine (terminal ileum) and cord like thickening of mesenteric lymphnodes. Acid
fast staining of cytological smears prepared from the intestinal scrapings and mesenteric
lymphnode of all animals revealed presence of clusters of acid fast bacilli. Mesentric
lymphnode and intestine were collected from during postmortem examination and
subjected to polymerase chain reaction (PCR) using specific primers targeting IS 900 gene
sequence of MAP which revealed positivity to MAP infection. The study concluded that
postmortem examination combined with polymerase chain reaction is highly useful in
accurate diagnosis of JD in small ruminants.
Description
TNV_IJCMAS_2018_7(8)2360-2363
Keywords
Veterinary Science