Molecular analysis of antagonistic effect of Trichoderma to Fusarium in Tomato
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Date
2019
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Birsa Agricultural University, Ranchi
Abstract
Tomato (Solanum lycopersicum L.) is a widely grown vegetable crop acquiring
the status of world’s most popular vegetable due to its importance for nutritive value,
processing and export potential. Tomato is affected by many diseases among which wilt
caused by Fusarium oxysporum is one of the important disease, resulting in 10-50 per
cent yield loss around the world. Fusarium oxysporum is a serious soil-borne pathogen
and persists for longer periods in the soil.
Plant treated with Trichoderma viride and Fusarium oxysporum at different time
interval. The result revealed that plant treated with Trichoderma viride have altered
metabolic activities as they undergo a stress condition. As per our analysis by visual
observation the 5 day Trichoderma treated plant with recovery are found more healthy
which shows the antagonistic effect of Trichoderma viride against Fusarium oxysporum.
This could be due to the beneficial effect of TV in promoting plant growth.
Molecular estimation was also performed. DNA extracted from Tomato,
Trichoderma viride and Fusarium oxysporum amplified with OPA series primers. And
result revealed that primer OPA 13 can distinguish between Tomato, Trichoderma viride
and Fusarium oxysporum.
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Description
Molecular analysis of antagonistic effect of
Trichoderma to Fusarium in Tomato