“EVALUATION OF BIOFILM OF AEROMONAS HYDROPHILA FOR ORAL VACCINATION OF AMUR COMMON CARP (CYPRINUS CARPIO HAEMATOPTERUS)”
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Date
2019
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
Biofilm of Aeromonas hydrophila oral vaccination was used to evaluate the effect on growth, antibody titre, hematological, immunological responses and disease resistance to A. hydrophila in Amur common carp fingerlings. 60 days trial with Biofilm, free cell and control diet in triplicates was conducted vaccinated for a period of 20 days (fed with vaccinated feed) and remaining 40 days were fed with control diet. Results indicated that SGR, mean weight gain, ADG, PER was significantly higher (p < 0.05) and FCR was best in the biofilm vaccinated group of fish as compared to that of free cell vaccine and control. No mortality was observed during the experimental period. Biofilm vaccine had a significant effect (p < 0.05) on the hematological, antibody response, immunological and serum biochemical indices. RBC, WBC, thrombocytes, hemoglobin and PCV was significantly higher (p < 0.05) in the biofilm vaccinated groups, there was no significant difference (p > 0.05) was found in MCV, MCH and MCHC between the control, free cell and biofilm vaccine. Biofilm vaccinated fish had a higher (p < 0.05) antibody response than other groups. Super oxide anion production, lysozyme activity and serum protein was found to be significantly higher (p < 0.05) in the biofilm vaccinated group but serum glucose and cholesterol was significantly lower (p < 0.05) in the biofilm vaccinated group. Biofilm vaccinated group of fish showed higher RPS (87%) than free cell vaccinated group of fish (40%).