EXPRESSION OF AQUAPORINS IN MAMMARY GLAND AND SKIN OF MURRAH BUFFALOES DURING DIFFERENT SEASONS
| dc.contributor.advisor | SINGH, MAHENDRA | |
| dc.contributor.author | AJITHAKUMAR H M | |
| dc.date.accessioned | 2023-11-10T14:11:37Z | |
| dc.date.available | 2023-11-10T14:11:37Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Present study experiment was conducted to determine the relative gene expression of aquaporins in different stages of buffalo mammary gland and skin tissue with heat stress. Mammary gland samples were obtained from New Delhi slaughter house during summer season. The results of the present study confirmed presence of AQP1, AQP3 and AQP5 in the mammary gland by immunolocalization and RT-PCR and AQP3 in BuMEC under in vitro studies. The relative expression of AQP3 in BuMEC was significantly higher (P<0.01) due to hyperosmotic stress 7% lactose over the 5% lactose, heat (42 oC) and NaCl treatment. Experiment 2 on restricted (twice a day) and ad lib (5 times a day) water intake on AQP gene expression (AQP3 and AQP5) in Murrah buffaloes during summer season revealed water Intake, plasma Na, ADH and ANG II was significantly (p<0.01) higher in ad lib feeding group as compared to restricted water intake. Experiment 3 on lactating Murrah buffaloes during hot dry and hot humid season indicated increase in DMI, body weight, milk production and fat content (p<0.01) by using with mist and fan cooling in hot dry season and fan cooling in hot humid season due to treatment by decreasing values of physiological responses. Plasma sodium concentration was significantly (p<0.01) higher cooling group as compared to control group buffaloes. Mist and fan downregulated (p<0.01) AQP3 and AQP5 gene expressions during hot dry season, however expression was upregulated in fan provided group in hot humid season. Plasma ADH, ANG II was lower (p<0.01) and ALD was higher in mist and fan group. It was concluded that higher expression of AQPs ( AQP1, AQP3 and AQP5) in lactating mammary gland tissue regulate milk secretion further hyperosmotic stress of lactose was more potent to induce AQP3 expression in BuMEC. Increasing THI upregulates the relative mRNA expression of AQP3 and AQP5. The relative lower expression of AQP3 and AQP5 mRNA in the mist and fan treatment group as compared to control during hot dry season indicated stress on animals and there by high expression occurred to cope up with the heat stress. The lower expression of AQP3 and AQP5 indicated lower evaporative loss at high humidity in control as compared to fan treatment group. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810200954 | |
| dc.language.iso | English | |
| dc.pages | 176 p. | |
| dc.publisher | ICAR-NDRI, KARNAL | |
| dc.sub | Animal Physiology | |
| dc.theme | Ph.D. | |
| dc.these.type | Ph.D | |
| dc.title | EXPRESSION OF AQUAPORINS IN MAMMARY GLAND AND SKIN OF MURRAH BUFFALOES DURING DIFFERENT SEASONS | |
| dc.type | Thesis |