EXPRESSION OF AQUAPORINS IN MAMMARY GLAND AND SKIN OF MURRAH BUFFALOES DURING DIFFERENT SEASONS
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Date
2019
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Publisher
ICAR-NDRI, KARNAL
Abstract
Present study experiment was conducted to determine the relative gene
expression of aquaporins in different stages of buffalo mammary gland and skin
tissue with heat stress. Mammary gland samples were obtained from New Delhi
slaughter house during summer season. The results of the present study
confirmed presence of AQP1, AQP3 and AQP5 in the mammary gland by
immunolocalization and RT-PCR and AQP3 in BuMEC under in vitro studies. The
relative expression of AQP3 in BuMEC was significantly higher (P<0.01) due to
hyperosmotic stress 7% lactose over the 5% lactose, heat (42 oC) and NaCl
treatment. Experiment 2 on restricted (twice a day) and ad lib (5 times a day) water
intake on AQP gene expression (AQP3 and AQP5) in Murrah buffaloes during
summer season revealed water Intake, plasma Na, ADH and ANG II was
significantly (p<0.01) higher in ad lib feeding group as compared to restricted
water intake. Experiment 3 on lactating Murrah buffaloes during hot dry and hot
humid season indicated increase in DMI, body weight, milk production and fat
content (p<0.01) by using with mist and fan cooling in hot dry season and fan
cooling in hot humid season due to treatment by decreasing values of
physiological responses. Plasma sodium concentration was significantly (p<0.01)
higher cooling group as compared to control group buffaloes. Mist and fan
downregulated (p<0.01) AQP3 and AQP5 gene expressions during hot dry
season, however expression was upregulated in fan provided group in hot humid
season. Plasma ADH, ANG II was lower (p<0.01) and ALD was higher in mist and
fan group. It was concluded that higher expression of AQPs ( AQP1, AQP3 and
AQP5) in lactating mammary gland tissue regulate milk secretion further
hyperosmotic stress of lactose was more potent to induce AQP3 expression in
BuMEC. Increasing THI upregulates the relative mRNA expression of AQP3 and
AQP5. The relative lower expression of AQP3 and AQP5 mRNA in the mist and
fan treatment group as compared to control during hot dry season indicated stress
on animals and there by high expression occurred to cope up with the heat stress.
The lower expression of AQP3 and AQP5 indicated lower evaporative loss at high
humidity in control as compared to fan treatment group.