DETECTION OF IL-4 AND IFN-Y mRNA CYTOKINE SECRETION FROM SENSITIZED CAPRINE ANTIGEN PRESENTING CELLS INDUCED CULTURE BY SEMI-QUANTITATIVE RT-PCR
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Date
2008
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Abstract
RT-PCR technique had been chosen to study the caprine antigen presenting cells induced Th cells cytokine
secretion proļ¬le. Macrophages induced more IFN- Y than IL-4 cytokine mRNA and in contrast, B cells induced higher
IL-4 production than IFN-Y. In general, IL-4 mRNAs were more in two antigen pulsed cultures as assessed by integrated
densitometry analysis.
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Veterinary Science, Veterinary Microbiology