Induction of Programmed cell death in tobacco Bright Yellow-2 cell lines and Saccharomyces cerevisiae strainYPH-500 against various apoptotic stimuli
| dc.contributor.advisor | Mathew, M K | |
| dc.contributor.author | LAKSHMANA REDDY, DC | |
| dc.date.accessioned | 2017-02-25T04:12:29Z | |
| dc.date.available | 2017-02-25T04:12:29Z | |
| dc.date.issued | 2008-02 | |
| dc.description.abstract | Programmed cell death (PCD) is a fundamental cellular process conserved in metazoans, plants and yeast. Our results suggested that hydrogen peroxide, heat and salinity induces PCD in tobacco bright yellow-2 cell lines and yeast strain YPH 500; these results are comparable to earlier reports. In tobacco BY-2 cell lines, hydrogen peroxide induces programmed cell death at 10mM concentration and typical features of PCD like membrane blebbing, DNA fragmentation, ROS rise and MMP loss were observed. Vacuole morphology varied during PCD process against H2O2, heat and salinity stresses. l. PCD observed in yeast against H2O2, heat and salinity stresses with typical hallmarks like DNA fragmentation, ROS rise and MMP loss. The concentration of H2O2 required in yeast is lower than the tobacco cells. In case of salinity, yeast requires higher (1.5M) as against 200mM in tobacco cells; there is no difference in induction of PCD with respect to heat stress in both systems. The death percentages were reduced by pre incubating yeast cells with calcium and ROS chelator and scavengers respectively. In yeast cells, elevation of ROS levels was observed against NaCl and heat stress and the elevation of cytosolic calcium levels was observed against H2O2 and NaCl stress. The rise of calcium levels was reduced by pre incubation with EGTA. The rise of calcium levels is less in H2O2 stress compare to saline stress. The reduction in percentage of cell death was observed in yeast cells against H2O2, Heat and NaCl by preincubation with scavengers. TEMPOL and Glutathione reduce cell death against H2O2 and Heat, where as EGTA reduces cell death against H2O2, Heat and NaC In our study, results conclude, both tobacco BY-2 cell and yeast cells exhibit substantially similar PCD characters against H2O2, Heat and NaCl stresses | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810002503 | |
| dc.keywords | Induction of Programmed cell death in tobacco | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 180 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Induction of Programmed cell death in tobacco | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Induction of Programmed cell death in tobacco Bright Yellow-2 cell lines and Saccharomyces cerevisiae strainYPH-500 against various apoptotic stimuli | en_US |
| dc.type | Thesis | en_US |