Induction of Programmed cell death in tobacco Bright Yellow-2 cell lines and Saccharomyces cerevisiae strainYPH-500 against various apoptotic stimuli
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Date
2008-02
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Programmed cell death (PCD) is a fundamental cellular process conserved in
metazoans, plants and yeast. Our results suggested that hydrogen peroxide, heat and
salinity induces PCD in tobacco bright yellow-2 cell lines and yeast strain YPH 500;
these results are comparable to earlier reports. In tobacco BY-2 cell lines, hydrogen
peroxide induces programmed cell death at 10mM concentration and typical features of
PCD like membrane blebbing, DNA fragmentation, ROS rise and MMP loss were
observed. Vacuole morphology varied during PCD process against H2O2, heat and
salinity stresses.
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PCD observed in yeast against H2O2, heat and salinity stresses with typical
hallmarks like DNA fragmentation, ROS rise and MMP loss. The concentration of H2O2
required in yeast is lower than the tobacco cells. In case of salinity, yeast requires higher
(1.5M) as against 200mM in tobacco cells; there is no difference in induction of PCD
with respect to heat stress in both systems. The death percentages were reduced by pre
incubating yeast cells with calcium and ROS chelator and scavengers respectively.
In yeast cells, elevation of ROS levels was observed against NaCl and heat stress
and the elevation of cytosolic calcium levels was observed against H2O2 and NaCl stress.
The rise of calcium levels was reduced by pre incubation with EGTA. The rise of calcium
levels is less in H2O2 stress compare to saline stress. The reduction in percentage of cell
death was observed in yeast cells against H2O2, Heat and NaCl by preincubation with
scavengers. TEMPOL and Glutathione reduce cell death against H2O2 and Heat, where
as EGTA reduces cell death against H2O2, Heat and NaC
In our study, results conclude, both tobacco BY-2 cell and yeast cells exhibit
substantially similar PCD characters against H2O2, Heat and NaCl stresses
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