BIO-PHYSICAL, BIO-CHEMICAL CHARACTERS AND MOLECULAR CHARACTERISATION OF DIFFERENT CASTOR GENOTYPES FOR RESISTANCE TO LEAFHOPPER, Empoasca flavescens Fabricius.

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dc.contributor.advisorVENKATESWARLU, N.C.
dc.contributor.authorMOUNICA, B
dc.date.accessioned2018-08-13T08:06:40Z
dc.date.available2018-08-13T08:06:40Z
dc.date.issued2017
dc.descriptionD5511en_US
dc.description.abstractThe present study on "Bio-physical, bio-chemical characters and molecular characterisation of different castor genotypes for resistance to leafhopper, Empoasca flavescens Fabricius.” was carried out in Dryland farm of Sri Venkateswara Agricultural College and IFT, RARS, Tirupati during kharif, 2016-17. Screening of twenty eight castor genotypes against leafhopper E. flavescens revealed that the mean leafhopper population varied significantly among the genotypes and high population was recorded in DPC-9 (54.28/3 leaves) followed by DCH-177 (42.93). Least leafhopper population was recorded in GCH-7 (20.48), VP-1 (20.63). The genotypes DPC-9, PCH-111 and DCH-177 recorded highest hopper burn scores of 2.4, 2.4 and 2.0 respectively. Lowest hopper burn scores were recorded in genotypes GCH-7 (0.00), PCH-254 (0.00) and SKI-336 (0.00). Genotypes GCH-7, PCH-254 and SKI-336 could be ranked as the least preferred genotypes with lowest leafhopper population and hopper burn scores while the genotypes DPC-9, DCH-177 could be ranked as most preferred genotypes with highest leafhopper population and hopper burn scores. This could be supported by the fact that GCH-7, PCH-254 and SKI336 were triple bloom genotypes imparting resistance to the leafhopper by antixenotic mechanism while DPC-9, DCH-177 and PCH-111 were zero and single bloom genotypes, respectively with no wax bloom on their leaf lamina. The correlation studies on leafhopper population with abiotic factors showed that maximum temperature, minimum temperature, rainfall, number of rainy days and evaporation were negatively correlated whereas relative humidity and sunshine hours were positively correlated with leafhopper population. Leaf colour of different castor genotypes was recorded through SCMR at monthly intervals upto 90 DAS. The correlation studies of SCMR with leafhopper population and hopper burn scores in castor showed that there was a negative significant correlation between SCMR and both the leafhopper population and hopper burn scores. In the present investigation no trichomes were observed on castor leaves. Among bio-chemical components of castor, phenols had a significant negative correlation with both leafhopper population and hopper burn scores. Total carbohydrates showed a significant positive correlation with leafhopper population. Total free amino acids exhibited significant negative correlation with hopper burn scores. The yield of different genotypes ranged from 217.25 to 31.31g/ plant. Highest yield was recorded in SKI-336 (217.25 g/plant) followed by M-574 (174.44 g/ plant), PCH-254 (152.16 g/ plant) while the lowest yield was recorded in SKI-335 (31.31 g/ plant) followed by SKI-84 (38.85 g/ plant) DCH-519 (52.51 g/ plant) and DCS-107 (55.95 g/ plant). Twelve contrasting genotypes were selected out of twenty eight genotypes for studying the molecular diversity by RAPD and SSR techniques. Eleven random primers (OPA 01, 07, 15, 19, OPC 02, 07, 08, 15 OPD 07, 09, 10) were used for RAPD analysis and generated scorable PCR products by amplifying the template DNA with Taq polymerase. The RAPD products generated a total 155 fragments with an average of 14.09 fragments per primer and the fragments were found 100 per cent polymorphic. The number of scorable bands produced per primer ranged from 2 to 34 and size of the products ranged from 75 bp to 6900 bp. The phylogenetic relationship among 12 castor genotypes was analysed by UPGMA method. The cluster analysis showed three major clusters having GCH-4, PCH-111, Jwala, Pragathi in one cluster, DPC-9, SKI-336, GCH-7, VP-1 in one cluster and PCH-282, Kranthi, SKI-341, DCH-177 in another cluster. Molecular diversity was studied with a set of 41 microsatellite or SSR primers in twelve contrasting castor genotypes. The SSR markers generated a total 120 fragments with an average of 2.86 fragments per primer and the fragments were found 81.09 per cent polymorphic. The number of scorable bands produced per primer ranged from 1 to 5 and size of the products ranged from 85 bp to 880 bp. The phylogenetic relationship among 12 castor genotypes was analysed by UPGMA method. The dendrogram constructed showed three major clusters having DPC-9, SKI-336, GCH-7 in one cluster, PCH111,Pragathi, GCH-4, Jwala in one cluster and SKI-341, PCH-282, DCH177, Kranthi and VP-1 in another cluster. Out of 41 primers, six primers exhibited 0 per cent polymorphism, three primers exhibited 50 per cent polymorphism and remaining 32 primers exhibited 100 per cent polymorphismen_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810064213
dc.keywordsBIO-PHYSICAL, BIO-CHEMICAL, CASTOR, GENOTYPES, LEAFHOPPER, Empoasca flavescens Fabricius.en_US
dc.language.isoen_USen_US
dc.pages118en_US
dc.publisherAcharya N.G. Ranga Agricultural Universityen_US
dc.research.problemBIO-PHYSICAL, BIO-CHEMICAL CHARACTERS AND MOLECULAR CHARACTERISATION OF DIFFERENT CASTOR GENOTYPES FOR RESISTANCE TO LEAFHOPPER, Empoasca flavescens Fabricius.en_US
dc.subEntomologyen_US
dc.subjectnullen_US
dc.themeBIO-PHYSICAL, BIO-CHEMICAL CHARACTERS AND MOLECULAR CHARACTERISATION OF DIFFERENT CASTOR GENOTYPES FOR RESISTANCE TO LEAFHOPPER, Empoasca flavescens Fabricius.en_US
dc.these.typeM.Scen_US
dc.titleBIO-PHYSICAL, BIO-CHEMICAL CHARACTERS AND MOLECULAR CHARACTERISATION OF DIFFERENT CASTOR GENOTYPES FOR RESISTANCE TO LEAFHOPPER, Empoasca flavescens Fabricius.en_US
dc.typeThesisen_US
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