Canine Parentage Verification Using Microsatellite Markers
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Date
2018-09
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Journal ISSN
Volume Title
Publisher
Indian Veterinary Journal
Abstract
A study was designed to assess a panel of ten
microsatellite markers to determine parentage
in four representative pedigree breeds of dogs -
the Dalmatian, Dachshund, Labrador Retriever
and Shih-Tzu. The microsatellite loci of interest
were amplified by polymerase chain reaction
and the resulting augmented products were
run on a genetic analyser. Allele sizes, analysed
with the help of a gene mapping software, were
measured and compared, to determine similarities between the progeny and their putative
parents. Conclusively, the microsatellites were
found to be a reliable molecular tool in parent-
age analysis due to its uniform distribution
throughout the genome.
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Keywords
Veterinary Science