APPLICATION OF POLYMERASE CHAIN REACTION FOR DIAGNOSIS AND MOLECULAR EPIDEMIOLOGY OF ANTHRAX IN LIVESTOCK IN KARNATAKA SHASHIKALA, N.
| dc.contributor.advisor | Dr. SHRIKRISHNA ISLOOR | |
| dc.contributor.author | SHASHIKALA, N. | |
| dc.date.accessioned | 2021-02-25T09:29:40Z | |
| dc.date.available | 2021-02-25T09:29:40Z | |
| dc.date.issued | 2018-01 | |
| dc.description.abstract | The present study employed the microscopy and the PCR for the detection of B .anthracis by targeting virulence genes for protective antigen (PA) and capsule (CAP) located on two plasmids, pXO1 and pXO2 respectively. Further, Ba813 gene of B. anthracis and nhe gene of B. cereus were targeted for species specific identification. In all, 14 blood smears from 5 different locations (Bellary, Koppal, Davangere, Doddaballapura and Chamarajnagar) were subjected to Gram’s and Polychrome methylene blue (PMB) staining. Of these, 2 smears from Doddaballapura were negative for anthrax bacilli by both the staining techniques. These blood smears and the blood samples were also found negative by PCR. Further, out of 10 blood samples from Bellary, Koppal, Doddaballapura and Chamrajnagar, 5 were PCR positive. Out of 14 blood smears from Davanagere, Doddaballapura and Chamarajanagar, only 4 smears revealed PCR positivity. All the 5 ear peice samples collected from Bellary, Koppal and Tumkur were found negative by PCR. None of the 5 soil samples from Bellary, Koppal and Chamarajnagara were PCR positive. The B. cereus isolate showed amplification for both PA as well as nhe genes. Sequencing and phylogenetic analysis of the PA gene revealed that the PA gene sequences of the B. cereus were homologous to that of B. anthracis. This indicated close genetic relatedness between B. anthracis and B. cereus. The high genetic homology (98%) among B. anthracis isolates was revealed. The Ba813 and CAP gene based phylogenetic analysis indicated probable prevalence of two strains of B. anthracis in the outbreak of anthrax in Bellary. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810161660 | |
| dc.keywords | VETERINARY MICROBIOLOGY | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 100 | en_US |
| dc.publisher | KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR – 585 401 | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.theme | VETERINARY MICROBIOLOGY | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | APPLICATION OF POLYMERASE CHAIN REACTION FOR DIAGNOSIS AND MOLECULAR EPIDEMIOLOGY OF ANTHRAX IN LIVESTOCK IN KARNATAKA SHASHIKALA, N. | en_US |
| dc.type | Thesis | en_US |