Development of EST-SSR markers and assessment of genetic diversity, relationships and population structure in aonla (Phyllanthus emblica L
| dc.contributor.advisor | RAJNISH SHARMA | |
| dc.contributor.author | MEGHA SHARMA | |
| dc.date.accessioned | 2023-10-21T15:08:31Z | |
| dc.date.available | 2023-10-21T15:08:31Z | |
| dc.date.issued | 2023-06-05 | |
| dc.description.abstract | ABSTRACT Phyllanthus emblica L. (Aonla) is an important edible and non-timber forest product species which have numerous medicinal and therapeutic uses. In the present study, commercial and wild aonla genotypes were characterized on physico-chemical parameters and molecular parameters. EST-SSRs were developed from fruit transcriptome data as well as data retrieved from NCBI database and further subjected to assess the genetic diversity and population structure. The significant variations were observed in physico-chemical parameters (fruit size, weight, colour, TSS, acidity, ascorbic acid and total phenolic contents) among all studied aonla genotypes. There was a significant positive correlation observed among most of the studied fruit attributes. Principal component analysis (PCA) divided the studied traits into two principal components i.e. PC1 and PC2 that accounted for 71.69 of the total variability. The cluster analysis was performed using Ward’s method that divided the aonla genotypes into two major clusters i.e. A and B where cluster A grouped all the wild genotypes, while cluster B comprised of all the commercial varieties. Under molecular characterization, out of total 102 EST-SSR primers, 46 were found to be polymorphic and further used in diversity analysis. The allele number was found to be varied from 2 to 26 with an average of 11.28 allele per locus. PIC, EMR, MI and Rp values ranged from 0.24 to 0.94, 0.50 to 25.00, 0.12 to 23.50 and 1.45 to 18.09, respectively. The mean value of He and Ho was 0.64 and 0.74. The dendrogram divided the aonla genotypes into two main clusters at a similarity coefficient of 0.43. The two wild genotypes ‘HPU5’ and ‘HPU6’ had the maximum similarity coefficient i.e. 0.82. However, the genotypes ‘NA-6’ and ‘HPSo3’ were found to be highly diversified to each other. Population structure analysis showed admixture of four different genetic pools. Although the correlation between physicochemical and molecular parameters found to be moderate but both these methods revealed the high genetic diversity among studied aonla genotypes which can be explored in breeding programmes for selection of superior genotypes. Furthermore, developed polymorphic EST-SSRs in this study can be utilized in future to study the population genetics and genetic resource management of the Phyllanthus emblica and its closely related species. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810199629 | |
| dc.keywords | Aonla | |
| dc.keywords | diversity | |
| dc.keywords | Phyllanthus | |
| dc.keywords | EST-SSRs | |
| dc.keywords | physico-chemical | |
| dc.keywords | molecular characterization | |
| dc.language.iso | English | |
| dc.pages | 136+ii | |
| dc.publisher | UHF,NAUNI | |
| dc.sub | Biotechnology | |
| dc.theme | Development of EST-SSR markers and assessment | |
| dc.these.type | Ph.D | |
| dc.title | Development of EST-SSR markers and assessment of genetic diversity, relationships and population structure in aonla (Phyllanthus emblica L | |
| dc.type | Thesis |