IN VITRO SELECTION AND BIOCHEMICAL CHARACTERIZATION Of CARNATION( DIANTHUS CARYOPHYLLUS l.) CALLUS CULTURE TOLERANT OF ALTERNARIA DIANTHI
| dc.contributor.advisor | NATH, A.K. | |
| dc.contributor.author | MEHTA, RUPALI | |
| dc.date.accessioned | 2018-07-31T07:37:45Z | |
| dc.date.available | 2018-07-31T07:37:45Z | |
| dc.date.issued | 2004 | |
| dc.description.abstract | Carnation (Dianthus caryophyllus L.) is one of the most important cut flowers of the world and commands a respectable status. With the increase in its cultivation the limiting factors in the production of quality flowers are also increasing Among the various fungal pathogens attacking this crop, Alternaria dianthi is known to cause leaf blight of carnation. Therefore, under present investigations attempts were made to produce resistant carnation calli and characterized them by using biochemical parameters. For this an efficient protocol for establi shment of callus culture and organogenesis were standardized. For callus establishment equal concent ration (2 mg/I) of NAA and ki netin was used and for regeneration of shoots from ca Iii 0.2 mg/I BAP and 0.5 mg/I NAA was used. For rooting liquid MS medium supplemented with 2 mg/I IBA and 0.2 per cent activated charcoal was found to be best. For in vilru selection technique di fferent concentrations (5-25%) of culture fi ltrate of Alternaria dianthi was used. Selective dose of culture fi ltrate was found to be 15 per cent \vhere l 1.67 per cent calli survived. The selected calli were cultured on medium devoid of culture fi ltrate for 4 weeks. Stability fo r resistance was checked by reculturing it on selective medium. Selected calli showed significantly higher phenol s, reducing sugars, total sugars and proteins. Difference in banding pattern of isoenzyme viz . est erase, peroxidase a.1d polyphenol oxidase was obtained in the selected call i | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810062750 | |
| dc.keywords | Carnation (Dianthus caryophyllus L.),Alternaria dianthi,DISEASES | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 52+XIII | en_US |
| dc.publisher | UHF,NAUNI | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Carnation (Dianthus caryophyllus L.),Alternaria dianthi,DISEASES | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | IN VITRO SELECTION AND BIOCHEMICAL CHARACTERIZATION Of CARNATION( DIANTHUS CARYOPHYLLUS l.) CALLUS CULTURE TOLERANT OF ALTERNARIA DIANTHI | en_US |
| dc.type | Thesis | en_US |