IN VITRO SELECTION AND BIOCHEMICAL CHARACTERIZATION Of CARNATION( DIANTHUS CARYOPHYLLUS l.) CALLUS CULTURE TOLERANT OF ALTERNARIA DIANTHI
Loading...
Files
Date
2004
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UHF,NAUNI
Abstract
Carnation (Dianthus caryophyllus L.) is one of the most important cut flowers
of the world and commands a respectable status. With the increase in its cultivation
the limiting factors in the production of quality flowers are also increasing Among
the various fungal pathogens attacking this crop, Alternaria dianthi is known to cause
leaf blight of carnation. Therefore, under present investigations attempts were made to
produce resistant carnation calli and characterized them by using biochemical
parameters. For this an efficient protocol for establi shment of callus culture and
organogenesis were standardized. For callus establishment equal concent ration (2
mg/I) of NAA and ki netin was used and for regeneration of shoots from ca Iii 0.2 mg/I
BAP and 0.5 mg/I NAA was used. For rooting liquid MS medium supplemented with
2 mg/I IBA and 0.2 per cent activated charcoal was found to be best. For in vilru
selection technique di fferent concentrations (5-25%) of culture fi ltrate of Alternaria
dianthi was used. Selective dose of culture fi ltrate was found to be 15 per cent \vhere
l 1.67 per cent calli survived. The selected calli were cultured on medium devoid of
culture fi ltrate for 4 weeks. Stability fo r resistance was checked by reculturing it on
selective medium. Selected calli showed significantly higher phenol s, reducing
sugars, total sugars and proteins. Difference in banding pattern of isoenzyme viz .
est erase, peroxidase a.1d polyphenol oxidase was obtained in the selected call i
Description
Keywords
null