Identification of Molecular Markers for Fusarium Wilt Resistance in Pigeonpea (Cajanus Cajan)
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Date
2012
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UAS Dharwad
Abstract
The investigation was carried out to identify the molecular markers linked to
Fusarium wilt resistance in pigeonpea and to identify the high yielding lines associated with
Fusarium wilt resistance. Cultivars, ‘ICP 8863’, a highly resistant genotype to Fusarium wilt
and ‘GS-1’ a highly susceptible genotype were used as parents to develop mapping
population. ‘ICP 8863’ and ‘GS-1’ were crossed during kharif 2007 and advanced to get F2
during kharif 2008. A total of 492 SSR markers were employed to assign the parental
polymorphism. Of them only two markers (P70 and P155) were found polymorphic. These
two markers were analysed through bulk segregant analysis in F2 in which SSR marker P70
confirmed its close linkage with trait of interest. Marker P70 was genotyped in RILs of 150
populations to validate the genetic linkage of the marker. The segregation pattern based on
both phenotypic value of the wilt reaction and marker data in RILs analysed through chisquare
test confirmed the expected results. High yielding pigeonpea RILs associated with
Fusarium wilt resistance were isolated from the experiments conducted at UAS, Dharwad
and ARS, Gulbarga during 2009-2012. It was possible to isolate transgressive wilt resistant
plants in F2 and high yielding resistant recombinant inbred lines (RILs) from RIL population
derived from ‘Maruti’ × ‘GS-1.’ Lines/plants were selected based on seed yield and yield
related traits such as 100 seed weight and no .of pods per plant. Phenotyping 288 F2 plants for
yield related traits and screening F2:3 for Fusarium wilt reaction helped to isolate 10
transgressive wilt resistant plants in F2 seed yield ranging from 169.15 to 217.78 g per plant
and 100 seed weight from 9.14 to 13.57 g. Further, two high yielding wilt resistant RILs, 109
and 243 were identified with seed yield of 15.33 and 13.65 q per ha and with seed weight of
10.08 and 11.19 g, respectively.