Identification of Molecular Markers for Fusarium Wilt Resistance in Pigeonpea (Cajanus Cajan)
| dc.contributor.advisor | P.M. Salimath | |
| dc.contributor.author | Nalini. A. Dharwad | |
| dc.date.accessioned | 2016-07-26T09:40:44Z | |
| dc.date.available | 2016-07-26T09:40:44Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | The investigation was carried out to identify the molecular markers linked to Fusarium wilt resistance in pigeonpea and to identify the high yielding lines associated with Fusarium wilt resistance. Cultivars, ‘ICP 8863’, a highly resistant genotype to Fusarium wilt and ‘GS-1’ a highly susceptible genotype were used as parents to develop mapping population. ‘ICP 8863’ and ‘GS-1’ were crossed during kharif 2007 and advanced to get F2 during kharif 2008. A total of 492 SSR markers were employed to assign the parental polymorphism. Of them only two markers (P70 and P155) were found polymorphic. These two markers were analysed through bulk segregant analysis in F2 in which SSR marker P70 confirmed its close linkage with trait of interest. Marker P70 was genotyped in RILs of 150 populations to validate the genetic linkage of the marker. The segregation pattern based on both phenotypic value of the wilt reaction and marker data in RILs analysed through chisquare test confirmed the expected results. High yielding pigeonpea RILs associated with Fusarium wilt resistance were isolated from the experiments conducted at UAS, Dharwad and ARS, Gulbarga during 2009-2012. It was possible to isolate transgressive wilt resistant plants in F2 and high yielding resistant recombinant inbred lines (RILs) from RIL population derived from ‘Maruti’ × ‘GS-1.’ Lines/plants were selected based on seed yield and yield related traits such as 100 seed weight and no .of pods per plant. Phenotyping 288 F2 plants for yield related traits and screening F2:3 for Fusarium wilt reaction helped to isolate 10 transgressive wilt resistant plants in F2 seed yield ranging from 169.15 to 217.78 g per plant and 100 seed weight from 9.14 to 13.57 g. Further, two high yielding wilt resistant RILs, 109 and 243 were identified with seed yield of 15.33 and 13.65 q per ha and with seed weight of 10.08 and 11.19 g, respectively. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/69825 | |
| dc.publisher | UAS Dharwad | en_US |
| dc.sub | Genetics and Plant Breeding | |
| dc.these.type | Ph.D | |
| dc.title | Identification of Molecular Markers for Fusarium Wilt Resistance in Pigeonpea (Cajanus Cajan) | en_US |
| dc.type | Thesis | en_US |