PHENOTYPIC AND GENOTYPIC DIVERSITY OF DIFFERENT GROUNDNUT (Arachis hypogaea L) ROOT NODULE BACTERIA (Rhizobium)

Simple item page
dc.contributor.advisorDr. V. P. Chovatia
dc.contributor.authorMr. B. J. Malviya
dc.date.accessioned2017-06-06T06:24:27Z
dc.date.available2017-06-06T06:24:27Z
dc.date.issued2010-01
dc.description.abstractAn investigation was carried out to study the diversity of groundnut Root Nodule Bacteria (RNB), isolated from different areas of Junagadh and Rajkot districts, using various phenotypic and molecular techniques. Twenty four RNB isolates of groundnut, comprising 21 RNB isolates from different areas of Junagadh and Rajkot districts, were studied with three standard strains of Rhizobium, collected from National Research Centre for Groundnut (NRCG), Junagadh. The 21 RNB isolates were selected and confirmed as Rhizobium, using several biochemical tests and the pure cultures were preserved on YEMA slants for further study. All the strains were analyzed for phenotypic tests, namely, pH tolerance, salt tolerance, antibiotics resistance profile and carbohydrate utilization pattern. All the RNB isolates and standard strains grew well at the pH 6.5 to 8.5. Most of RNB isolates were able to tolerate higher NaCl but were unable to tolerate higher CaCl2. Out of eleven antibiotics tested, carbenicillin, erythromycin, gentamicin and streptomycin could differentiate RNB isolates into different groups; while polymyxin-B, ampicillin, tetracycline and chloramphenicol were found ineffective in grouping the RNB isolates. All RNB isolates and standard strains were able to utilize most of carbon sources used here. In plant infection (nodulation) test, carried out in vitro (pot study) with groundnut cultivars (GG-20 and TAG-24) and the RNB isolates, viz JND-3, JND-5, JND-9, JND-13, RJ-4, RJ-7 and IGR-6 revealed positive results for GG-20. While JND-1, JND-3, JND-9, JND-10, RJ-2 and TAL-100 revealed positive results for TAG-24. The isolates JND-9, RJ-5, JND-12 were found superior for root length, shoot length and fresh weight in GG-20. While the isolates JND-3, JND-9, JND-13 were found superior for root length, shoot length and fresh weight in TAG-24. The 16S rDNA PCR-RFLP assay clustered all the isolates into four groups. Majority of RNB isolates were distributed district wise in dendogram. Two strain JND-10 and JND-11 were grouped separately, confirming the phylogenetic relationship. IGR-6 was grouped with RNB isolates of Junagadh district. While TAL-1000 was grouped with RNB isolates of Rajkot districts. The nif H gene amplification revealed only single desired fragment in all RNB isolates and standard strains (approximately 781 bp). BOX-PCR clustered all RNB isolates and standard strains into five groups. All the RNB isolates of group I were similar to standard strains TAL-1000 and IGR-6, while all the isolates of group III were similar to standard strain NC-92.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810018267
dc.language.isoenen_US
dc.pages128en_US
dc.publisherjau,junagadhen_US
dc.subAgricultural Biotechnologyen_US
dc.subjectbiotechnologyen_US
dc.themePHENOTYPIC AND GENOTYPIC DIVERSITY OF DIFFERENT GROUNDNUT (Arachis hypogaea L) ROOT NODULE BACTERIA (Rhizobium)en_US
dc.these.typeM.Scen_US
dc.titlePHENOTYPIC AND GENOTYPIC DIVERSITY OF DIFFERENT GROUNDNUT (Arachis hypogaea L) ROOT NODULE BACTERIA (Rhizobium)en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1339.pdf
Size:
52.29 MB
Format:
Adobe Portable Document Format
Description:
PHENOTYPIC AND GENOTYPIC DIVERSITY OF DIFFERENT GROUNDNUT (Arachis hypogaea L) ROOT NODULE BACTERIA (Rhizobium)
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thesis