EFFECT OF SUPPLEMENTATION OF GLUTATHIONE AND α-TOCOPHEROL IN TRIS AND SKIM MILK BASED EXTENDERS FOR PRESERVATION OF RAM SEMEN AT REFRIGERATION TEMPERATURE
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Date
2019-11
Journal Title
Journal ISSN
Volume Title
Publisher
KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The research findings of the present study are that addition of glutathione improved
motile sperm and reduced immotile sperm percentage than α-tocopherol in skim milk than
TRIS egg yolk based extenders. Addition of glutathione improved rapid progressive sperm
percentage than α-tocopherol in TRIS egg yolk than skim milk based extenders. Addition
of α-tocopherol in TRIS egg yolk reduced slow progressive sperm percentage whereas
improved in skim milk based extenders as compared to addition of glutathione. Addition
of either glutathione or α-tocopherol did not reduce the non-progressive sperm percentage
in skim milk and TRIS egg yolk based extenders. Addition of glutathione improved sperm
curvilinear velocity, sperm average path velocity and sperm straight line velocity in TRIS
egg yolk based extenders whereas α-tocopherol reduced it in skim milk based extenders.
Addition of glutathione did not improve sperm straightness percentage in skim milk and
TRIS egg yolk based extenders however addition of α-tocopherol reduced it in TRIS egg
yolk than skim milk based extenders. Addition of α-tocopherol reduced sperm wobble
percentage in skim milk than TRIS egg yolk based extenders at refrigeration however
glutathione has shown no effect on it. Addition of glutathione in skim milk based extenders
improved sperm beat cross frequency, sperm dance and sperm dance mean whereas
addition of α-tocopherol in TRIS egg yolk extender reduced it. There was no effect of
addition of glutathione on amplitude of lateral sperm head displacement in skim milk based
extender however addition of α-tocopherol improved it at 72 h of refrigeration in NARI
Suwarna rams.