Anthrax vaccine 34F2 Sterne strain growth in nutrient agar and Soyabean casein digest agar

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dc.contributor.authorParthiban, S.
dc.contributor.authorMammen, Lancy
dc.contributor.authorKalaivani, S.R.
dc.contributor.authorSundrapandian, V.
dc.contributor.authorRahman, A. Habibur
dc.contributor.authorTANUVAS
dc.date.accessioned2018-09-07T11:58:03Z
dc.date.available2018-09-07T11:58:03Z
dc.date.issued2012
dc.description.abstractThe most widely used strain for anthrax vaccine production is 34F2 Sterne strain. The aim of the study was to determine a suitable solid media for the cultivation of 34F2 Sterne strain for large scale vaccine production. Seed material (34F2 Sterne strain) was cultured in 70 Roux flasks each containing Nutrient agar and Soyabean casein digest agar separately and allowed for sporulation. The spores were washed with sterile saline and the pure spore suspensions were pooled and weighed separately. The weight of the spore suspension was 1.801 kg and 1.830 kg for Nutrient agar and Soyabean casein digest agar respectively. The number of live spore was 1:250 in 1 ml of 10^-7 dilution for both Nutrient agar and Soyabean casein digest agar grown spore suspensions. The spore weight and spore count are almost equal for the both the solid media but based on cost and quantity of medium required the Nutrient agar is preferred over Soyabean casein digest agar.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810072417
dc.keywordsVeterinary Science, Veterinary Microbiology, Anthrax, 34F2 Sterne strain, Growth, Nutrient agar, Soyabean casein digest agaren_US
dc.language.isoen_USen_US
dc.pages284en_US
dc.relation.ispartofseries;04
dc.subjectVeterinary Scienceen_US
dc.subjectVeterinary Microbiologyen_US
dc.titleAnthrax vaccine 34F2 Sterne strain growth in nutrient agar and Soyabean casein digest agaren_US
dc.typeArticleen_US
dc.volume16en_US
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