Anthrax vaccine 34F2 Sterne strain growth in nutrient agar and Soyabean casein digest agar
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Date
2012
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Abstract
The most widely used strain for anthrax vaccine production is 34F2 Sterne strain. The aim
of the study was to determine a suitable solid media for the cultivation of 34F2 Sterne
strain for large scale vaccine production. Seed material (34F2 Sterne strain) was cultured
in 70 Roux flasks each containing Nutrient agar and Soyabean casein digest agar
separately and allowed for sporulation. The spores were washed with sterile saline and
the pure spore suspensions were pooled and weighed separately. The weight of the
spore suspension was 1.801 kg and 1.830 kg for Nutrient agar and Soyabean casein
digest agar respectively. The number of live spore was 1:250 in 1 ml of 10^-7 dilution for
both Nutrient agar and Soyabean casein digest agar grown spore suspensions. The spore
weight and spore count are almost equal for the both the solid media but based on cost
and quantity of medium required the Nutrient agar is preferred over Soyabean casein
digest agar.
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Keywords
Veterinary Science, Veterinary Microbiology