Studies on Differentially Expressed Genes for Fibre Development in Diploid Cotton (Gossypium arboretum L.)
| dc.contributor.advisor | I. S. Katageri | |
| dc.contributor.author | Atul Suresh Hande | |
| dc.date.accessioned | 2016-11-23T17:29:58Z | |
| dc.date.available | 2016-11-23T17:29:58Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | A global gene expression profiling study at initiation and elongation stages of fibre development in Gossypium arboreum L. was undertaken to identify key genes using Fuzzylinted and Fuzzy-lintless lines. Scanning electron microscopy revealed no difference in fibre initials but showed difference at elongation stage. Overall 278 transcripts were differentially expressed during fibre initiation and elongation stage in fuzzy-lintless line. The network covers range of transcription factors like AP2-EREBP, C2H2, and WRKY up-regulated at 0 dpa and down-regulated at 10 in Fl line and similarly phytohormones like abscisic acid, auxin, brassinosteroid, ethylene, gibberellin, and salicylic acid expressed differentially. Transcripts coding TPS, UGE, EXPANSINs, AGPs, BGAL13, UGT74B1, TUBs, ACO and LTPs coding for energy and cell wall metabolism were down-regulated at 10 dpa in Fl line. Down-regulation of transcripts related to signal transduction like RLKs, LRR-family protein, Ca2+ and ROS resulted to the loss of co-ordination during the 0 dpa. Down-regulation of miscellaneous factors having role in stress response and cell growth like HSPs and SPDS3 at elongation stage suggested probable role in cell elongation. In SSH study, based on the putative functions of genes expressed in preferentially fibre elongation stage in forward library of fuzzy-linted line included ADF2, ACO1, -galactosidase, -glucanase, -tubulin, XTH32/XET32, EF1, CBL, CaM, PCY and PLC. About 155 novel genes having no similarity in the database were also found in this study. Attempt was made for validation of the same set of genes through quantitative real-time PCR which showed high correlations with the expression levels in morphology, microarray and SSH. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/87285 | |
| dc.publisher | UAS, Dharwad | en_US |
| dc.research.problem | Studies on Differentially Expressed Genes for Fibre Development in Diploid Cotton (Gossypium arboretum L.) | en_US |
| dc.sub | Agricultural Biotechnology | en_US |
| dc.these.type | M.Sc | |
| dc.title | Studies on Differentially Expressed Genes for Fibre Development in Diploid Cotton (Gossypium arboretum L.) | en_US |
| dc.type | Thesis | en_US |