Studies on Differentially Expressed Genes for Fibre Development in Diploid Cotton (Gossypium arboretum L.)
Loading...
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UAS, Dharwad
Abstract
A global gene expression profiling study at initiation and elongation stages of fibre
development in Gossypium arboreum L. was undertaken to identify key genes using Fuzzylinted
and Fuzzy-lintless lines. Scanning electron microscopy revealed no difference in fibre
initials but showed difference at elongation stage. Overall 278 transcripts were differentially
expressed during fibre initiation and elongation stage in fuzzy-lintless line. The network
covers range of transcription factors like AP2-EREBP, C2H2, and WRKY up-regulated at 0
dpa and down-regulated at 10 in Fl line and similarly phytohormones like abscisic acid,
auxin, brassinosteroid, ethylene, gibberellin, and salicylic acid expressed differentially.
Transcripts coding TPS, UGE, EXPANSINs, AGPs, BGAL13, UGT74B1, TUBs, ACO and
LTPs coding for energy and cell wall metabolism were down-regulated at 10 dpa in Fl line.
Down-regulation of transcripts related to signal transduction like RLKs, LRR-family protein,
Ca2+ and ROS resulted to the loss of co-ordination during the 0 dpa. Down-regulation of
miscellaneous factors having role in stress response and cell growth like HSPs and SPDS3 at
elongation stage suggested probable role in cell elongation. In SSH study, based on the
putative functions of genes expressed in preferentially fibre elongation stage in forward
library of fuzzy-linted line included ADF2, ACO1, -galactosidase, -glucanase, -tubulin,
XTH32/XET32, EF1, CBL, CaM, PCY and PLC. About 155 novel genes having no
similarity in the database were also found in this study. Attempt was made for validation of
the same set of genes through quantitative real-time PCR which showed high correlations
with the expression levels in morphology, microarray and SSH.