STUDY OF GENES INVOLVED IN SALT TOLERANCE DURING EARLY GROWTH STAGES IN KHARCHIA LOCAL WHEAT

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dc.contributor.advisorKanika
dc.contributor.authorRAKESH BHOWMICK
dc.date.accessioned2016-09-23T19:06:08Z
dc.date.available2016-09-23T19:06:08Z
dc.date.issued2012
dc.descriptionT-8602en_US
dc.description.abstractPresent study was conducted to screen the genes induced by salinity stress at two different growth stages (seedling and tillering) in Kharchia Local wheat. Physiological analysis was carried out in both salt tolerant Kharchia local and salt susceptible HD2009. The study revealed that Kharchia local maintained better physiological state as compared to HD2009 during salinity stress. Subtractive cDNA libraries were constructed from two different developmental stages (seedling and tillering stages). Two hundred and eighty eight recombinant clones were sequenced from each library. Sixteen percent of the genes identified in seedling stage and twenty five percent in tillering stage were related to stress. These included catalase, superoxide dismutase, glutathion reductase, WRKY 80 transcription factors, aldehyde dehydrogenase etc. Some genes like translationally controlled tumor protein, poly A binding protein, senescence associated proteins were also induced by salinity treatment. More than 25% of the ESTs generated fell in unknown/no hit category upon homology search through BLAST analysis. Differential expression of three genes was confirmed by RT PCR analysis. Key Words: Suppression Subtractive Hybridizatioen_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/78685
dc.publisherIARI, NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGYen_US
dc.subMolecular Biology and Biotechnology
dc.subjectplanting, livestock, rice, biological phenomena, productivity, group communication, crops, diseases, yields, economic systemsen_US
dc.these.typeM.Sc
dc.titleSTUDY OF GENES INVOLVED IN SALT TOLERANCE DURING EARLY GROWTH STAGES IN KHARCHIA LOCAL WHEATen_US
dc.typeThesisen_US
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