STUDY OF GENES INVOLVED IN SALT TOLERANCE DURING EARLY GROWTH STAGES IN KHARCHIA LOCAL WHEAT
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Date
2012
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Publisher
IARI, NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY
Abstract
Present study was conducted to screen the genes induced by salinity stress at
two different growth stages (seedling and tillering) in Kharchia Local wheat.
Physiological analysis was carried out in both salt tolerant Kharchia local and salt
susceptible HD2009. The study revealed that Kharchia local maintained better
physiological state as compared to HD2009 during salinity stress. Subtractive cDNA
libraries were constructed from two different developmental stages (seedling and
tillering stages). Two hundred and eighty eight recombinant clones were sequenced
from each library. Sixteen percent of the genes identified in seedling stage and
twenty five percent in tillering stage were related to stress. These included catalase,
superoxide dismutase, glutathion reductase, WRKY 80 transcription factors,
aldehyde dehydrogenase etc. Some genes like translationally controlled tumor
protein, poly A binding protein, senescence associated proteins were also induced by
salinity treatment. More than 25% of the ESTs generated fell in unknown/no hit
category upon homology search through BLAST analysis. Differential expression of
three genes was confirmed by RT PCR analysis.
Key Words: Suppression Subtractive Hybridizatio
Description
T-8602
Keywords
planting, livestock, rice, biological phenomena, productivity, group communication, crops, diseases, yields, economic systems