MOLECULAR CHARACTERIZATION OF LETTUCE (Lactuca sativa L.) GENOTYPES USING RAPD-PCR
| dc.contributor.advisor | SRIVASTAVA, D.K. | |
| dc.contributor.author | SHARMA, SHUBHANGI | |
| dc.date.accessioned | 2017-05-11T12:35:11Z | |
| dc.date.available | 2017-05-11T12:35:11Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Abstract The present studies on ‘‘Molecular characterizationof lettuce (Lactuca sativa L.) genotypes using RAPD” was carried out to study the genetic relationship among various genotypes. The aim of this study was to investigate genetic relationship among different genotypes of lettuce. 25 lettuce genotypes namely 1-(CGN 10944-2), 2-(CGN-14651), 3-(CGN-05169), 4-(UHF-Sel.-07), 5-(CGN 10944-3), 6-(CGN-05198), 7-(UHF-Sel.-03), 8-(CGN-05167), 9-(CGN-20721), 10-(CGN-04987), 11-(CGN 10944-1), 12-(CGN-04990), 13-(CGN-04933), 14-(CGN-04934), 15-(CGN-14629), 16-(UHF-Sel.-04), 17-(UHF-Sel.-04), 18-(CGN-19009), 19-(CGN-09373), 20-(CGN-11358), 21-(Great lakes), 22-(CGN-19088), 23-(CGN-04543), 24-(UHFSel.-01) and 25-(UHF-Sel.-02) were selected for the study. A totalof 45 primers were tried to generate RAPD profile, out of these reproducible patterns were obtained with 22 primers. A total of 87 bands were obtained out of which all were polymorphic and seven primers have shown unique bands with the specific genotypes. The percentage of total polymorphism was 100% in RAPD. Similarity index was computed based on Jacquard coefficient and used for cluster analysis based on UPGMA. RAPD technology could be useful for identification of different accessions as well as accessing the genetic similarity among different genotypes of lettuce. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810010986 | |
| dc.keywords | lettuce (Lactuca sativa L.) ,genotypes using RAPD,Molecular characterization | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | UHF,NAUNI | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | lettuce (Lactuca sativa L.) ,genotypes using RAPD,Molecular characterization | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | MOLECULAR CHARACTERIZATION OF LETTUCE (Lactuca sativa L.) GENOTYPES USING RAPD-PCR | en_US |
| dc.type | Thesis | en_US |