MOLECULAR CHARACTERIZATION OF LETTUCE (Lactuca sativa L.) GENOTYPES USING RAPD-PCR
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Date
2013
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Publisher
UHF,NAUNI
Abstract
Abstract
The present studies on ‘‘Molecular characterizationof lettuce (Lactuca sativa
L.) genotypes using RAPD” was carried out to study the genetic relationship among
various genotypes. The aim of this study was to investigate genetic relationship
among different genotypes of lettuce. 25 lettuce genotypes namely 1-(CGN 10944-2),
2-(CGN-14651), 3-(CGN-05169), 4-(UHF-Sel.-07), 5-(CGN 10944-3), 6-(CGN-05198), 7-(UHF-Sel.-03), 8-(CGN-05167), 9-(CGN-20721), 10-(CGN-04987), 11-(CGN 10944-1), 12-(CGN-04990), 13-(CGN-04933), 14-(CGN-04934), 15-(CGN-14629), 16-(UHF-Sel.-04), 17-(UHF-Sel.-04), 18-(CGN-19009), 19-(CGN-09373),
20-(CGN-11358), 21-(Great lakes), 22-(CGN-19088), 23-(CGN-04543), 24-(UHFSel.-01) and 25-(UHF-Sel.-02) were selected for the study. A totalof 45 primers were
tried to generate RAPD profile, out of these reproducible patterns were obtained with
22 primers. A total of 87 bands were obtained out of which all were polymorphic and
seven primers have shown unique bands with the specific genotypes. The percentage
of total polymorphism was 100% in RAPD. Similarity index was computed based on
Jacquard coefficient and used for cluster analysis based on UPGMA. RAPD
technology could be useful for identification of different accessions as well as
accessing the genetic similarity among different genotypes of lettuce.
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