Elucidation of phytoconstituents and antioxidant & in vitro antiinflammatory activities of nine members of family Lamiaceae
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Date
2019-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Medicinal herbs offer an important traditional way to prevent and cure several diseases. Various anti-inflammatory drugs have been used to reduce pain and inflammation. However, there are many side effects associated with non- steroidal drugs. Therefore, a search for non-toxic natural compounds with antioxidant activity has intensified in recent years. In the present study, nine wild plant species of family Lamiaceae were selected to assess their antioxidant and anti-inflammatory potential. The extraction yield (%) was maximum in Pogostemon benghalensis (21.01) and Vitex negundo (18.71) in aqua-methanol and aqua-acetone, respectively. All the phytochemicals are present in all the species except saponins present in Clerodendrum infortunatum, Colebrookea oppositifolia and V. negundo; terpenoids were absent in Roylea elegans. In both aqua-methanol and aqua-acetone, the values in different plant species was maximum in C. oppositifolia in terms of DPPH (80.17 and
70.35 %), FRAP (109.38 and 83.72, μg BHTE/mg), FCA (57.1 and 54.55 μg BHTE/mg) and TAA (116.13 and 69.95 μg VCE/mg) assays, respectively. The TPC (μg GAE/mg) was maximum in C. oppositifolia (36.10) and P. benghalensis (30.20) in aqua-methanol and aqua-acetone, respectively
while the TFC ((μg QE/mg) was maximum in V. negundo (41.93 and 44.266) in both aqua-methanol and aqua-acetone. The IC50 values (μg/ml) of DPPH were comparatively higher in R. elegans (409.0) in aqua-methanol and Lamium amplexicaule (178.97) in aqua-acetone and low in C. oppositifolia (26.17 and 41.08 in aqua-methanol and aqua-acetone, respectively); and IC50 values of FCA ranged from 106.13 (C. oppositifolia) to 594.69 (V. negundo) in aqua-methanol; and 111.608 (C. oppositifolia) to 676.48 (R. elegans) in aqua-acetone. Anti-inflammatory potential against protein denaturation (%) was comparatively higher in C. oppositifolia in both aqua-methanol (73.93) and aqua-acetone (64.78) than other species. Among the solvents, aqua-methanol showed comparatively higher antioxidant activity than aqua-acetone. Positive and significant correlation in terms of FRAP, DPPH, FCA, TAA and TPC was obtained in all species. Thus, the above mentioned plant species
with comparatively higher antioxidant and anti-inflammatory potential than the others and can be a potent natural plant source of nutritional and anti-inflammatory drugs which can be utilized for developing natural curatives against synthetic ones. It can be exploited in nutraceutical and pharmaceutical industries.
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