HISTOLOGY, HISTOCHEMISTRY AND ULTRASTRUCTURE OF HEART, LIVER AND PANCREAS IN PIGS (Sus scrofa domesticus)

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dc.contributor.advisorDr. K.V. JAMUNA
dc.contributor.authorBHARATH KUMAR, M. L.
dc.date.accessioned2021-02-05T09:30:30Z
dc.date.available2021-02-05T09:30:30Z
dc.date.issued2018-09
dc.description.abstractThe histology, histochemistry and ultrastructure of heart, liver and pancreas of pigs was studied. Epicardium was composed of mesothelium which was simple squamous epithelium resting on basement membrane, myocardium predominated with cardiac muscle fibres along with connective tissue and Purkinje fibres while endocardium was lined by endothelium beneath which there was subendothelial tissue. Histochemically myocardium showed least presence of neutral and acidic mucopolysaccharide, while epithelium and endothelium gave intense positive reaction. The coronary artery was made of three layers namely, tunica intima, tunica media and tunica adventitia of which tunica media was thicker composed of smooth muscle fibres interlaced with reticular fibres while tunica adventitia composed of loose connective tissue rich in elastic fibres. The moderator band was categorised into regions with the presence of cardiomyocytes and Purkinje fibres while endothelium acted as a capsule. Histologically valves had lamina fibrosa, lamina spongiosa and lamina atrialis/ ventricularis composed predominantly with collagen fibres, histiocytes and elastic fibres respectively. The papillary muscle and chordae tendinae junction was clearly demarcated and distinguished with abundant number of Purkinje fibres. Histochemically, various intensities of reactions of acid mucopolysaccharides and glycosaminoglycans in different regions of all the tissues were observed. The liver had capsule which entered the parenchyma and divided into different hexagonal shaped hepatic lobules which had central vein in the centre and radiating hepatic lamina composed of hepatocyes bordering sinusoids. Sinusoids had space of Disse lined with endothelium, Kupffer cells and Ito cells. Bile canaliculi between two hepatocytes were evident both histologically and ultrastructurally. Hepatocytes reacted differently to polysaccharides while capsule and interlobular septa showed intense positive reaction. Reticular fibres which was fine, branching and anastomosing framed the hepatocytes and sinusoids. The pancreas was tubuloacinar type of gland covered by a thin capsule, made up of predominantly collagen fibres. Each acinus showed a single row of pyramidal epithelial cells resting on the basement membrane. The cytoplasm of the acinar cells showed two distinct zones i.e., basal and apical zone which was basophilic and acidophilic with numerous zymogen granules. Three types of acinar cells were noted in pancreas i.e. active, resting and exhausted type. The duct system of the pancreas consisted of larger interlobular, medium sized intralobular and small intercalated ducts. The endocrine tissue appeared as lightly stained areas between the darkly stained acini of different shapes and sizes. The alpha cells had pale cytoplasm, ovoid nucleus occupied the peripheral region in the islet but beta cells with dark cytoplasm and spherical nucleus were in the centre. Delta cells were distributed mainly peripherally in the periphery of islets and they were irregular in shape with cytoplasmic process. Key words: Pig, Heart, Liver, Pancreas, Valves, Myocardium, Hepatocytes, Islets.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810160927
dc.keywordsVETERINARY ANATOMY AND HISTOLOGYen_US
dc.language.isoEnglishen_US
dc.pages263en_US
dc.publisherKARNATAKA VETERINARY ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDARen_US
dc.subVeterinary Anatomy and Histologyen_US
dc.themeVETERINARY ANATOMY AND HISTOLOGYen_US
dc.these.typeM.V.Sc.en_US
dc.titleHISTOLOGY, HISTOCHEMISTRY AND ULTRASTRUCTURE OF HEART, LIVER AND PANCREAS IN PIGS (Sus scrofa domesticus)en_US
dc.typeThesisen_US
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