NDIRECT REGENERATION OF Japonica RICE (Oryza sativa L. spp. japonica) VARIETIES AZUCENA AND MOROBEREKAN THROUGH ANTHER CULTURE
| dc.contributor.advisor | Ashok, T. H. | |
| dc.contributor.author | AVINASH SHARMA | |
| dc.date.accessioned | 2020-10-07T08:57:27Z | |
| dc.date.available | 2020-10-07T08:57:27Z | |
| dc.date.issued | 2015-12-01 | |
| dc.description.abstract | Rice (Oryza sativa L.) is one of the most important food crops of Southeast Asia, which feeds half of the world population. Anther culture is an efficient and convenient technique for rapid production of doubled haploids which are useful in crop breeding programs. Present study involved evaluation of two japonica rice varieties Azucena and Moroberekan cultured on 17 callus induction treatments on N6 medium and 19 regeneration treatments on MS medium. Highest callus induction frequency was observed in Azucena in treatment T4 (2, 4-D 2 mg L-1 + Kinetin 1 mg L-1 ) (49.99 %) followed by Moroberekan in treatment T11 (2, 4-D 1 mg L-1 + NAA 2 mg L-1 + Kinetin 0.5 mg L-1 ) (48.64 %). Highest shoot regeneration frequency was recorded in Azucena in treatment T10 (Kinetin 0.5 mg L-1 + BAP 2 mg L-1 + NAA 1 mg L-1 ) (83.33 %) followed by Moroberekan in treatment T16 (Kinetin 2 mg L-1 + BAP 1 mg L-1 + NAA 1 mg L-1 ) (80.0 %). Plantlet regeneration did not occur in Azucena. Highest plantlet regeneration frequency was recorded in Moroberekan in treatment T16 (Kinetin 2 mg L-1 + BAP 1 mg L -1 + NAA 1 mg L-1 ) (80.0 %) followed by in treatment T10 (Kinetin 0.5 mg L-1 + BAP 2 mg L-1 + NAA 1 mg L-1 ) (55.55 %). These results suggest that growth regulators and their concentration, along with genotype have significant effect on callus induction and regeneration from anthers in rice. | en_US |
| dc.identifier.other | Th-11282 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810152767 | |
| dc.keywords | Rice,Moroberekan, Treatment, Varieties Azucena. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 109 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.theme | VARIETIES AZUCENA AND MOROBEREKAN THROUGH ANTHER CULTURE | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | NDIRECT REGENERATION OF Japonica RICE (Oryza sativa L. spp. japonica) VARIETIES AZUCENA AND MOROBEREKAN THROUGH ANTHER CULTURE | en_US |
| dc.type | Thesis | en_US |