NDIRECT REGENERATION OF Japonica RICE (Oryza sativa L. spp. japonica) VARIETIES AZUCENA AND MOROBEREKAN THROUGH ANTHER CULTURE
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Date
2015-12-01
Authors
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Publisher
UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU
Abstract
Rice (Oryza sativa L.) is one of the most important food crops of Southeast Asia,
which feeds half of the world population. Anther culture is an efficient and convenient
technique for rapid production of doubled haploids which are useful in crop breeding
programs. Present study involved evaluation of two japonica rice varieties Azucena and
Moroberekan cultured on 17 callus induction treatments on N6 medium and 19
regeneration treatments on MS medium. Highest callus induction frequency was
observed in Azucena in treatment T4 (2, 4-D 2 mg L-1
+ Kinetin 1 mg L-1
) (49.99 %)
followed by Moroberekan in treatment T11 (2, 4-D 1 mg L-1
+ NAA 2 mg L-1
+ Kinetin
0.5 mg L-1
) (48.64 %). Highest shoot regeneration frequency was recorded in Azucena in
treatment T10 (Kinetin 0.5 mg L-1
+ BAP 2 mg L-1
+ NAA 1 mg L-1
) (83.33 %) followed
by Moroberekan in treatment T16 (Kinetin 2 mg L-1
+ BAP 1 mg L-1
+ NAA 1 mg L-1
)
(80.0 %). Plantlet regeneration did not occur in Azucena. Highest plantlet regeneration
frequency was recorded in Moroberekan in treatment T16 (Kinetin 2 mg L-1
+ BAP 1 mg
L
-1
+ NAA 1 mg L-1
) (80.0 %) followed by in treatment T10 (Kinetin 0.5 mg L-1
+ BAP 2
mg L-1
+ NAA 1 mg L-1
) (55.55 %). These results suggest that growth regulators and
their concentration, along with genotype have significant effect on callus induction and
regeneration from anthers in rice.