CHARACTERIZATION OF GROWTH HORMOME (GH) GENE IN SHEEP BREEDS OF TAMIL NADU

dc.contributor.authorCauveri, D.
dc.contributor.authorSivaselvam, S.N.
dc.contributor.authorKarthickeyan, S.M.K.
dc.contributor.authorTirumurugaan, K.G.
dc.contributor.authorKumanan, K.
dc.contributor.authorTANUVAS
dc.date.accessioned2018-07-25T10:49:45Z
dc.date.available2018-07-25T10:49:45Z
dc.date.issued2017-10
dc.descriptionTNV_IJSR_2017_23(2)153-158en_US
dc.description.abstractTo identify the polymorphisms in the native sheep breeds, characterization of growth hormone (GH) gene was carried out in eight breeds (Coimbatore, Kilakarsal, Madras Red, Mecheri, Nilagiri, Ramnad White, Tiruchy Black and Vembur) of Tamil Nadu. To amplify the whole GH gene, four primers that generated overlapping amplicons of a mean size of 762 bp were used. The PCR products from a minimum of 12 samples from each breed were sent for sequencing. Both the forward and reverse sequences obtained in the FASTA and *.ab1 format were compared with the reference sequence from NCBI (NC_019468 and GeneID: 443329) for identifying the SNPs. Three novel SNPs 480 G>A (SNP-G1), 871 G>A (SNP-G2) and 1259 A>C (SNP-G3) were identified in Intron 1, 2 and 3 of GH gene, respectively. In Intron 1, a 480 G>A (SNP-G1) transition was found in all the eight breeds. All the three genotypes viz. GG, GA and AA were found in Madras Red, Mecheri, Nilagiri and Tiruchy Black, whereas Coimbatore, Kilakarsal, Ramnad White and Vembur showed only GG and GA genotypes. SNP-G2, a G>A transition was specific to Nilagiri sheep. In all the other breeds only AA genotype was present (reference G). In Nilagiri the G allele was found as a heterozygote in 17% of the animals screened. No GG animals were identified. SNP-G3, an A>C transversion was identified in the Intron 3 region of Coimbatore, Kilakarsal, Madras Red and Ramnad White breeds. In Kilakarsal and Madras Red all three genotypes (AA, AC and CC) were found, whereas Coimbatore sheep had AA and AC genotypes and Ramnad White had only AC genotype. It was concluded that the exons of GH gene were highly conserved and did not show any polymorphism whereas the intons were polymorphic.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810062003
dc.keywordsGH gene, Sheep, Single nucleotide polymorphism, Tamil Naduen_US
dc.language.isoenen_US
dc.pages153-158en_US
dc.publisherIndian Society for Sheep and Goat Production and Utilizationen_US
dc.relation.ispartofseries;2
dc.subjectVeterinary Scienceen_US
dc.subjectSmall Ruminantsen_US
dc.titleCHARACTERIZATION OF GROWTH HORMOME (GH) GENE IN SHEEP BREEDS OF TAMIL NADUen_US
dc.title.alternativeIndian Journal of Small Ruminantsen_US
dc.typeArticleen_US
dc.volume23en_US
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