CHARACTERIZATION OF GROWTH HORMOME (GH) GENE IN SHEEP BREEDS OF TAMIL NADU
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Date
2017-10
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Society for Sheep and Goat Production and Utilization
Abstract
To identify the polymorphisms in the native sheep breeds, characterization of growth hormone
(GH) gene was carried out in eight breeds (Coimbatore, Kilakarsal, Madras Red, Mecheri, Nilagiri,
Ramnad White, Tiruchy Black and Vembur) of Tamil Nadu. To amplify the whole GH gene, four
primers that generated overlapping amplicons of a mean size of 762 bp were used. The PCR
products from a minimum of 12 samples from each breed were sent for sequencing. Both the
forward and reverse sequences obtained in the FASTA and *.ab1 format were compared with the
reference sequence from NCBI (NC_019468 and GeneID: 443329) for identifying the SNPs.
Three novel SNPs 480 G>A (SNP-G1), 871 G>A (SNP-G2) and 1259 A>C (SNP-G3) were
identified in Intron 1, 2 and 3 of GH gene, respectively. In Intron 1, a 480 G>A (SNP-G1) transition
was found in all the eight breeds. All the three genotypes viz. GG, GA and AA were found in Madras
Red, Mecheri, Nilagiri and Tiruchy Black, whereas Coimbatore, Kilakarsal, Ramnad White and
Vembur showed only GG and GA genotypes. SNP-G2, a G>A transition was specific to Nilagiri
sheep. In all the other breeds only AA genotype was present (reference G). In Nilagiri the G allele
was found as a heterozygote in 17% of the animals screened. No GG animals were identified.
SNP-G3, an A>C transversion was identified in the Intron 3 region of Coimbatore, Kilakarsal,
Madras Red and Ramnad White breeds. In Kilakarsal and Madras Red all three genotypes (AA,
AC and CC) were found, whereas Coimbatore sheep had AA and AC genotypes and Ramnad
White had only AC genotype. It was concluded that the exons of GH gene were highly conserved
and did not show any polymorphism whereas the intons were polymorphic.
Description
TNV_IJSR_2017_23(2)153-158
Keywords
Veterinary Science, Small Ruminants