Quantification and initial profiling of salivary and cervico vaginal fluid proteins in murrah buffalos during estrus and proestrus using sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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dc.contributor.authorKavitha, K
dc.contributor.authorSuresh Kumar, R
dc.contributor.authorKrishnakumar, K
dc.contributor.authorSarath, T
dc.contributor.authorVijayarani, K
dc.contributor.authorArunmozhi, N
dc.contributor.authorBalasubramaniyan, S
dc.contributor.authorLeena, KB
dc.contributor.authorAbhinaya, K
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-11T06:53:03Z
dc.date.available2020-03-11T06:53:03Z
dc.date.issued2020-02
dc.descriptionTNV_20thMVC_PP_Feb-2020_YSA05en_US
dc.description.abstractSaliva and cervico vaginal fluid (CVF) are considered good non invasive biomarkers for detection of estrus in buffalo as external signs are not significant in these animals. Based on the linkage between salivary proteins and estrus, the present study was aimed to profile the salivary and CVF proteins in Murrahbuffaloes (atproestrus and estrus) using single dimension (1-D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).It was observed that the saliva collected from sevenestrus buffalo had proteins with the molecular weight of 26kDa, 31kDa, 50kDa, 70kDa and 76kDa whereas saliva from eight proestrus buffalo had proteins with molecular weight of 48kDa, 62kDa, 70kDa and 75kDa. Based on published data by Shashikumaret al., (2018) the proteins in the estrus and proestrus correspond to short palate lung and nasalepithelium carcinoma-associated protein 2B (26.5kDa), tRNA-methyltransferase catalytic subunit TRM61A, CCDC85A protein (50kDa), Heat shock 70kDa protein (70kDa), Testin (48kDa), Max-binding protein MNT and Rho guanine nucleotide exchange factor 37 (76.1kDa) or Serine/threonine-protein phosphatese 2A 65kDa regulatory subunit A beta isoform (76.7kDa). Molecular weight of proteins observed in the CVF collected during estrus (n=4) where predominantly of 70kDa and 78kDa which correspond to Heat shock 70kDa protein and Receptor-type tyrosine-protein phosphatase epsilon (78.49kDa) respectively based on the published data by Muthukumaret al.(2014). Thus these findings provide a lead for further analysis and evaluation of the proteins for estrus detection in buffalo.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810145162
dc.keywords20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVASen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleQuantification and initial profiling of salivary and cervico vaginal fluid proteins in murrah buffalos during estrus and proestrus using sodium dodecyl sulfate-polyacrylamide gel electrophoresisen_US
dc.title.alternativeNational Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safetyen_US
dc.typePresentationen_US
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