Quantification and initial profiling of salivary and cervico vaginal fluid proteins in murrah buffalos during estrus and proestrus using sodium dodecyl sulfate-polyacrylamide gel electrophoresis
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Date
2020-02
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Abstract
Saliva and cervico vaginal fluid (CVF) are considered good non invasive biomarkers for detection of
estrus in buffalo as external signs are not significant in these animals. Based on the linkage between salivary
proteins and estrus, the present study was aimed to profile the salivary and CVF proteins in Murrahbuffaloes
(atproestrus and estrus) using single dimension (1-D) sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SDS-PAGE).It was observed that the saliva collected from sevenestrus buffalo had proteins
with the molecular weight of 26kDa, 31kDa, 50kDa, 70kDa and 76kDa whereas saliva from eight proestrus
buffalo had proteins with molecular weight of 48kDa, 62kDa, 70kDa and 75kDa. Based on published data by
Shashikumaret al., (2018) the proteins in the estrus and proestrus correspond to short palate lung and nasalepithelium carcinoma-associated protein 2B (26.5kDa), tRNA-methyltransferase catalytic subunit TRM61A,
CCDC85A protein (50kDa), Heat shock 70kDa protein (70kDa), Testin (48kDa), Max-binding protein MNT
and Rho guanine nucleotide exchange factor 37 (76.1kDa) or Serine/threonine-protein phosphatese 2A 65kDa
regulatory subunit A beta isoform (76.7kDa). Molecular weight of proteins observed in the CVF collected
during estrus (n=4) where predominantly of 70kDa and 78kDa which correspond to Heat shock 70kDa protein
and Receptor-type tyrosine-protein phosphatase epsilon (78.49kDa) respectively based on the published data
by Muthukumaret al.(2014). Thus these findings provide a lead for further analysis and evaluation of the
proteins for estrus detection in buffalo.
Description
TNV_20thMVC_PP_Feb-2020_YSA05
Keywords
Veterinary Science