Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine
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Date
2017
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Abstract
Progesterone is a potent drug for synchronization of the estrus and ovulation cycles in bovine. At present, the
estrus cycle of bovine is controlled by the insertion of progesterone-embedded silicone bands. The disadvantage
of nondegradable polymer inserts is to require for disposal of these bands after their use. The study currently
focuses on preparation of biodegradable progesterone-incorporated nanofiber for estrus synchronization. Three different
concentrations (1.2, 1.9, and 2.5 g) of progesterone-impregnated nanofibers were fabricated using electrospinning. The
spun membrane were characterized by scanning electron microscopy, X-ray diffraction, differential scanning calorimetry,
thermogravimetric analysis, and Fourier transform infrared spectroscopy. Uniform surface morphology, narrow
size distribution, and interaction between progesterone and zein were confirmed by SEM. FTIR spectroscopy
indicated miscibility and interaction between zein and progesterone. X-ray analysis indicated that the size of zein
crystallites increased with progesterone content in nanofibers. Significant differences in thermal behavior of
progesterone-impregnated nanofiber were observed by DSC. Cell viability studies of progesterone-loaded nanofiber were
examined using MTT assay. In vitro release experiment is to identify the suitable progesterone concentration for estrus
synchronization. This study confirms that progesterone-impregnated nanofibers are an ideal vehicle for progesterone
delivery for estrus synchronization of bovines.
Description
TNV_NRL_2017_12(116)1-6
Keywords
Veterinary Science