SEROPREVALENCE OF BRUCELLOSIS IN CATTLE AND THEIR ATTENDANTS IN AND AROUND RANCHI
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Date
2010
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Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
The present study was undertaken to access the seroprevalence of
brucellosis in cattle and their attendants in and around Ranchi. The
serological tests, like STAT, RBPT, HIT, 2MET and ELISA were employed on
the collected serum samples of cattle and humans of occupationally exposed
persons like animal handler’s, farmers and patients having pyrexia of
unknown origin to detect Brucella antibodies. The MRT was also done after
the collection of milk samples from individual cow.
A total of 545 serum samples comprising 428 from cattle and 117 from
human beings collected from different areas of Ranchi district were
subjected to STAT, RBPT, HIT, 2MET and ELISA for detecting antibodies.
STAT was carried out by using Brucella abortus antigen and RBPT was
carried out by using RBPT antigen. Both antigens were procured from I.V.R.I
izzatnagar, Bareilly. Milk ring test was also done by using ABR antigen
supplied from I.V.R.I, izzatnagar Bareilly.
ELISA was carried out by using the smooth lipopolysacharide (s-lps)
based avidin-biotin ELISA kit supplied by project Director on animal disease
monitoring and surveillance (PD-ADMAS), Bangalore.
Out of 353 milk samples tested from individual cow comprising of
R.V.C dairy (16), Military dairy (214), Kisan dairy (78) and Local cattle (45)
87 (24.66%) were found positive. Out of 87 positive samples 4(25.00%),
45(21.03%), 20(25.64%), and 18(40%), were positive from R.V.C dairy,
Military dairy, Kisan dairy and Local cattle respectively.
In cattle a total of 428 serum samples comprising of 33, 220, 80 and
95 from R.V.C dairy, Military dairy, Kisan dairy and Local cattle were tested
by STAT, RBPT, HIT, 2-MET ELISA respectively. The seropositivity of
brucellosis were 4(12.12%), 36(16.36%), 16(20.00%) and 25(26.32%) in
STAT, 2(6.06%), 46(20.91%), 20(25.00%) and 28(29.47%) in RBPT, 0(0.00%),
26(11.82%), 13(16.25%) and 20(21.05%) in HIT, 0(0.00%), 24(10.91%),
11(13.75%) and 15(15.79%) in 2-MET and 2(6.06%), 49(22.27%), 22(27.50%)
and 30(31.58%) in ELISA in respective dairy cattle.
In human Out of 117 serum samples the seropositivity was recorded
8 (6.84%), 9(7.69%), 3(2.56%), 5(4.27%) and 10 (8.55 %) in STAT, RBPT, 2-
MET, HIT and ELISA respectively. Out of 117 serum samples 17 were of
animal handlers, 55 of farmers and 45 of patient having pyrexia of unknown
origin were tested by STAT, RBPT, 2-MET, HIT and ELISA for Brucella
agglutinins. The seropositivity was recorded 2(11.76%), 1(1.82%) and
5(11.11%) in STAT, 2(11.76%), 1(1.82%) and 6(13.33%) in RBPT, 1(5.88%)
0(0%) and 2(4.44%) in 2-MET, 1(5.88%) , 0(0.00%) and 4(8.89%), in HIT and
2(11.76%), 2(3.64%) and 6(13.33%) in ELISA respectively.
Out of 428 serum samples of cattle 37 were of retention of placenta,
26 of repeat breeders, 8 of abortion and 357 of apparently healthy cows
tested by STAT, RBPT, HIT, 2-MET and ELISA. The seropositivity was
21(56.76%), 16(61.54%), 7(87.50%), and 37(10.36%), in STAT, 23(62.16%),
16(61.54%), 8(100%) and 49(13.73%), in RBPT, 18(48.65%), 12(46.15%),
7(87.50%), and 22(6.16%), in HIT, 13(35.14%), 11(42.31%), 7(87.50%), and
19 (5.32%) in 2-MET and 24(64.86%) 19(73.08%) 8(100%) and 52(14.57%) in
ELISA respectively.
In cattle the relative sensitivity and specificity of RBPT and STAT were
also compared with ELISA. The RBPT showed 91.26 % sensitivity and 99.38
% specificity and STAT showed 77.66 % sensitivity and 99.69 % specificity in
cattle.
In human also RBPT and STAT was compared with ELISA for
sensitivity and specificity. The RBPT showed 90.00 % sensitivity and 100.00
% specificity, STAT showed 70.00 % sensitivity and 99.06 % specificity.
On the basis of above findings in present study the following points
may be concluded.
Description
SEROPREVALENCE OF BRUCELLOSIS IN CATTLE AND THEIR ATTENDANTS IN AND AROUND RANCHI
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