SYNERGISTIC EFFECT OF BIO-SYNTHESIZED SILVER NANOPARTICLES WITH SOME BIOCONTROL AGENTS AGAINST Spilarctia obliqua (Walker) INFESTING GREENGRAM
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Date
2023-07
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G. B. Pant University of Agriculture & Technology, Pantnagar-263145
Abstract
Nanotechnology for plant protection is an emerging research field that offers new
methods to amid nanomaterials as Nanopesticide. The present study was conducted to
evaluate the compatibility of AgNPs with egg Parasitoids, Trichogramma chilonis,
Entomopathogenic Nematode, Steinernema abbasi strain PN-01 and Entomopathogenic
fungus, Beauveria bassiana along with study on Synergistic effect of AgNPs with
Beauveria bassiana alone and in combination against Spilarctia obliqua under laboratory
and net house conditions. The biosynthesis of Silver nanoparticles was performed with
neem leaves extract and its characterization was done with UV-Vis spectrophotometer,
DLS, SEM and FTIR. The shape of the NPs was observed to be spherical and the average
size was 66.63 nm. It was observed that at highest concentration of AgNPs (40 ppm), there
was only 47.67 percent Adults of T. chilonis emerges from the eggs shell and 51.27 percent
reduction in eggs hatching over control was recorded at 3rd day after treatment. The Probit
data analysis shows that S abbasi was sensitive to AgNPs with LC50 0.37 ppb. In
compatibility test, all the concentrations of AgNPs were found compatible and harmless
except 20 ppm and 40 ppm for the growth of B. basssina as per Hassan’s classification
index. The LC50 and LT50 values recorded under laboratory condition for combination of
AgNPs and B. bassiana was 11.16 ppm and 77.31 hours, respectively against 6 days old
larvae of S. obliqua. The combination was found 2.85 times more toxic than AgNPs alone.
Under net house conditions, 100 percent probit mortality of S. obliqua was recorded on 8th
day after treatment when combination was used, while 42.85 and 53.57 percent probit
mortality was recorded for individual treatment of B. bassiana and AgNPs (40 ppm). This
study reveals that AgNPs with biological control agents turn to be promising cradle of
nontoxic pesticides that shows synergistic effect in controlling noxious insect pests. Further,
this technique will be a valuable tool in the integrated pest management system.
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Theses of M.Sc.