CULTURAL AND MORPHOLOGICAL CHARACTERIZATIONS OF Beauveria bassiana.
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Date
2019
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College of Agriculture Nagpur, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola
Abstract
An investigation entitled "CULTURAL AND MORPHOLOGICAL CHARACTERIZATIONS OF Beauveria bassiana" was carried out during 2018-2019. Effects of nutrition, pH, temperature and humidity were assessed on the basis of radial growth. Influence of different spore concentrations viz., 105, 106, 107, 108 and 109 spores per ml were tested for the pathogenicity of B. bassiana against second instar larvae of Spodoptera litura. Similarly compatibility of B. bassiana was evaluated with commonly used insecticides viz., spinosad 45 SC, imidachloprid 200 SL, chloropyriphos 20 EC and indoxacarb 14.5 SC.
The mummified bodies of lepidopteran pest larvae with
symptoms of fungal infection were collected from field conditions from different places. Detailed morphological studies of effective isolate (Bb3) show that the fungus grew well on SDA (sabouraud dextrose agar). The mycelium on this medium, appeared white, dense and powdery. The colonies appeared raised in the center and developed radial furrows. The culture was turned yellowish white and powder type. Spore structures was globose to sub-globose.
Two different semi synthetic media namely sabouraud dextrose agar and potato dextrose agar were evaluated for better growth of B. bassiana and it was observed that SDA medium (41.50 mm) support the growth of isolate. Significantly higher radial growth was however, seen in media on the basis of these results, pH value 6.0, temperature of 25°C and 95 per cent relative humidty was considered as optimum for growth and sporulation of B. bassiana.
In terms of larval mortality, 107 spores per ml was recorded highest mortality of S. litura followed by 108 spores per ml, least larval mortality was observed at 105 spores per ml after 3, 5, 7 and 10 days of treatment. Compatibility of B. bassiana against commonly used insecticides in plant protection was tested. Imidachloprid were non-toxic to B. bassiana, as no significant reduction in radial growth was noticed. The insecticide chloropyriphos recorded 77.20 per cent inhibition of growth and was toxic to B. bassiana.
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WARGANE, VILAS SUKHADEO. (2019). Cultural and morphological characterizations of Beauveria bassiana. Department of plant pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2019. Print. xi, 57p. (unpublished).