STUDIES ON SEASONAL VARIATION OF BIOACTIVE COMPOUNDS IN Salvia spp.
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Date
2023-11-28
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UHF Nauni
Abstract
The present investigation entitled “Studies on seasonal variation of bioactive compounds in Salvia spp.” was carried out in the field and laboratory of the Department of Forest Products, College of Forestry, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) during 2022-23. The study was conducted under two objectives namely (i) To standardize the method of extraction for rosmarinic acid (ii) To study the seasonal variation in rosmarinic acid and other bioactive compounds with growth and development of Salvia rosmarinus Spenn. and Salvia sclarea L. For standardization of extraction methods in Salvia rosmarinus and Salvia sclarea three different extraction methods viz. soxhlet extraction, reflux extraction and sonication assisted extraction were used with four different solvents. In S. rosmarinus maximum rosmarinic acid (2.23%) content was obtained in soxhlet extraction for six hours duration with methanol-water (70:30) and highest total extract (%) (40.66%) was recorded in soxhlet extraction for eight hours duration with methanol-water (70:30). In S. sclarea maximum rosmarinic acid content (1.55%) was obtained in soxhlet extraction for six hours duration with methanol-water (70:30) and highest total extract (%) (36.04%) was recorded in soxhlet extraction for ten hours duration with methanol-water (70:30). Monthly variation in rosmarinic acid was studied in four germplasms of rosemary viz. SS1, SS2, SS3 and SS4. On the basis of seasonal variation, maximum rosmarinic acid content (SS1-1.33%, SS2-2.42%, SS3-2.51% and SS4-2.23) in S. rosmarinus was recorded in the month of November and minimum rosmarinic acid content (SS1-0.21%, SS2-1.34%, SS3-1.37% and SS4-0.41%) was recorded in the month of July in all four germplasms. In S. sclarea maximum rosmarinic acid content (1.55%) was observed in the month of April (vegetative stage) and minimum (0.004%) was recorded in the month of July (seed setting stage). Total phenolic, flavonoid content and anti-oxidant activity in S. rosmarinus was highest in November and lowest in July in all four germplasms. In S. sclarea, total phenolic, flavonoid content and anti-oxidant activity was maximum in April and minimum in month of July. Simple and easy HPLC method was developed for quantification of rosmarinic acid in S. rosmarinus and S. sclarea.