BIO-EFFICACY AND GENOME ANALYSIS OF INDIGENOUS Bacillus thuringiensis ISOLATES AGAINST GROUNDNUT BRUCHID, Caryedon serratus (Olivier)
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Date
2024-02-19
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UNIVERSITY OF AGRICULTURAL SCINCES BANGALORE
Abstract
Bacillus thuringiensis, a soil bacterium serves as a biocontrol agent against various members of insect orders. Efficacy of B. thuringiensis against the coleopteran pest groundnut bruchid, Caryedon serratus (Olivier) remains seldom documented. Understanding the genetic diversity of B. thuringiensis and its entomopathogenicity against C. serratus would be a viable option to manage C. serratus. In the present study, a total of twenty-five B. thuringiensis isolates were isolated from soil and deceased larvae of Holotrichia serrata. Bioassay of these isolates against second instar larvae of C. serratus yielded an efficient B. thuringiensis strain NBAIR-BtHS with the pest mortality rate of 68
% and recorded significantly lower LC50 value (15.96 μg/mL) compared to control. Whole genome sequencing of this isolate through Illumina next generation platform revealed the presence of 5.5 Mbp and 2.2 Mbp of genomic and plasmid DNA that contained 5,845 and
2,283 genes, respectively. Mining for novel insect toxicity related genes using BtToxin scanner revealed a total of 18 genomic DNA borne genes (chitinase C, two copies of zwa6, two copies of inhA1, four copies of zwa5A ,four copies of bmp1, enhancin, mpp46Ab1, vpb4Ca1 and spp1Aa1) and 6 plasmid borne genes (two copies of zwa5A , mpp46Ab1, vpb4Ca1, spp1Aa1 and bmp1). The current study provides genetic insights into the B. thuringiensis strain NBAIR-BtHS and documents the presence of novel insecticide toxicity related genes which can be used for the development of transgenic plants resistant to coleopteran insects in general and C. serratus in particular to prevent post-harvest loss of groundnut.,
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BIO-EFFICACY AND GENOME ANALYSIS, Bacillus thuringiensis ISOLATES AGAINST GROUNDNUT