ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF SALMONELLA FROM UNPASTEURIZED SUGARCANE JUICE
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Date
2021
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SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),
Abstract
ABSTRACT
NAME RUCHI BALIYAN
ID. NO. 4888
DEGREE MSc. BIOTECHNOLOGY
Title “ Isolation and biochemical characterization of Salmonella from
unpasteurized sugarcane juice.”
Advisor Dr. Purushottam
Fruit juice are extremely popular because of their nutritional worth, mineral richness and
vitamin content as well as their affordibility. A lack of knowledge about hygienic practices
procedures among vendors leaves goods such as fruit juices susceptible to higher bacterial
contamination levels. Increased intake of fruit juice has a direct positive impact on economic
status, but it may have detrimental impact as foodborne illness, outbreaks and spoilage issues
occur. Globally there are instances of people becoming sick from fruit juices owing to foodborne
illness. About 150-177 million people are expected to be affected by this illness by
2030 based on the no. of cases recorded in United States in 2001. Pathogens like
enterohaemorrhagic E.coli, Salmonella, etc have all been found in juices. Salmonella is a
Gram- negative, oxidase negative, rod- shaped bacterium belongs to the family
enterobacteriaceae. It is a intracellular pathogen which can grow and reproduce inside the
host cell. Salmonella leads to systemic disease in human body. The role of Salmonella in
human illness has been recognized virtually since its discovery in 1885. Therefore, this study
is planned out to evaluvate the presence of Salmonella in unpasteurized sugarcane juice.
Which were collected from different regions of Shamli and Meerut. The samples were
inoculated into T.T broth and incubated at 37° C for 24 hrs followed by streaking on
MacConkey agar plates and then BSA plates followed by biochemical characterization. Total
15 samples out of 34 samples found to be positive for Salmonella as they display non-lactose
fermenting white colony on BSA, and blue- green colour colony on HEA. A series of
biochemical tests was performed, results of these test confirms the presence of Salmonellain
the samples. The isolates showed sensitivity to levofloxacin, netillin, and norfloxacin. The
zone of inhibition was 20 mm, 10 mm, and 20 mm respectively. And the isolates showed
resistance to a no. of antibiotics like ampicillin, imipenam, ceftazidime, cefixime, etc.
Antimicrobial effect of Haldi was also tested and Haldi have a significant effect on
Salmonella, Hence, it is suggested that natural antimicrobials can be used as an alternate to
antibiotics. The Salmonella isolates were resistant to many antibiotics. Therefore there is a
need of measuring hygienic protocols and prescribed and limited use of antibiotics to prevent
the transfer of resistance gene among the pathogen population.
(Purushottam) Ruchi Baliyan
Advisor